Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar – 608 002, Tamilnadu, India.
Toxicol Mech Methods. 2013 Feb;23(2):77-85. doi: 10.3109/15376516.2012.720301. Epub 2012 Oct 3.
The present investigation explores the anticlastogenic effect of diosgenin on 7,12-dimethylbenz(a)anthracene (DMBA) treated clastogenesis. The frequency of bone marrow micronucleated polychromatic erythrocytes (MnPCEs), chromosomal aberrations (CA), deoxyribonucleic acid (DNA) damage as cytogenetic markers and the levels of lipid peroxidation by-products, activities of enzymatic antioxidant and the status of detoxification agents were performed to assess the anticlastogenic effects of diosgenin on DMBA treated hamsters. Intraperitoneal injection of DMBA (30 mg/kg bw) leads to clastogenesis in hamster. Elevated MnPCEs frequencies, CA, DNA damage, enhanced lipid peroxidation by products, declined antioxidant activities and detoxification cascade were observed in DMBA treated hamsters. Oral pretreatment with diosgenin (80 mg/kg bw) daily for a period of five days significantly reduced the frequency of MnPCEs, CA, DNA damage and normalized the levels of lipid peroxidation by products with increased activities of antioxidants and detoxification agents in DMBA alone treated hamsters. Outcome of the present study revealed that diosgenin has potent anticlastogenic effects on DMBA treated hamsters.
本研究探讨了薯蓣皂素对 7,12-二甲基苯并(a)蒽（DMBA）诱发的染色体断裂的抗断裂作用。通过骨髓微核多染红细胞（MnPCEs）、染色体畸变（CA）、脱氧核糖核酸（DNA）损伤作为细胞遗传学标志物以及脂质过氧化产物的水平、抗氧化酶活性和解毒剂的状态来评估薯蓣皂素对 DMBA 处理的仓鼠的抗断裂作用。腹腔注射 DMBA（30mg/kg bw）可导致仓鼠发生染色体断裂。在 DMBA 处理的仓鼠中，MnPCEs 频率升高、CA 增加、DNA 损伤、脂质过氧化产物增加、抗氧化酶活性下降和解毒剂状态下降。薯蓣皂素（80mg/kg bw）每天口服预处理 5 天，可显著降低 MnPCEs 的频率、CA、DNA 损伤，并使 DMBA 单独处理的仓鼠中的脂质过氧化产物水平正常化，同时增加抗氧化酶和解毒剂的活性。本研究结果表明，薯蓣皂素对 DMBA 处理的仓鼠具有较强的抗断裂作用。
