睡茄内酯A对7,12-二甲基苯并(a)蒽诱导的叙利亚金仓鼠骨髓遗传毒性的保护作用。
Protective role of withaferin-A on 7,12-dimethylbenz(a)anthracene-induced genotoxicity in bone marrow of Syrian golden hamsters.
作者信息
Panjamurthy K, Manoharan S, Menon Venugopal P, Nirmal Madhavan R, Senthil N
机构信息
Department of Biochemistry and Biotechnology, Faculty of Science, Raja Muthaiah Dental College and Hospital, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India.
出版信息
J Biochem Mol Toxicol. 2008 Jul-Aug;22(4):251-8. doi: 10.1002/jbt.20235.
The present study has investigated the antigenotoxic effect of withaferin-A, a steroidal lactone obtained from the roots and leaves of Withania somnifera, in 7,12-dimethylbenz(a)anthracene (DMBA)-induced genotoxicity. Measurement of the frequency of micronucleated polychromatic erythrocytes (MnPCEs) and chromosomal aberrations is used as cytogenetic endpoints. A single intraperitoneal injection of DMBA (30 mg/kg b.w.) to golden Syrian hamsters resulted in marked elevation in the frequency of MnPCEs and aberrations in the chromosomal structure. Hamsters pretreated with withaferin-A intraperitonealy 2 h before the injection of DMBA, significantly reduced the frequency of MnPCEs and chromosomal aberrations such as chromosomal break, gap, minute, and fragment. Our results thus demonstrated the antigenotoxic effect of withaferin-A in DMBA-induced genotoxicity in the bone marrow of golden Syrian hamsters.
本研究考察了从睡茄的根和叶中提取的甾体内酯——睡茄内酯A，对7,12 - 二甲基苯并(a)蒽（DMBA）诱导的遗传毒性的抗诱变作用。以多染性红细胞微核（MnPCEs）频率和染色体畸变的测定作为细胞遗传学终点。对金黄叙利亚仓鼠单次腹腔注射DMBA（30 mg/kg体重）导致MnPCEs频率显著升高以及染色体结构畸变。在注射DMBA前2小时腹腔注射睡茄内酯A预处理的仓鼠，显著降低了MnPCEs频率以及染色体断裂、裂隙、微小体和片段等染色体畸变。因此，我们的结果证明了睡茄内酯A对DMBA诱导的金黄叙利亚仓鼠骨髓遗传毒性具有抗诱变作用。
Zotero 插件