G1/S特异性细胞周期蛋白D1可能是喉鳞状细胞癌患者的一种预后生物标志物。

G1/S-specific cyclin-D1 might be a prognostic biomarker for patients with laryngeal squamous cell carcinoma.

作者信息

Zhang Ying-Yao, Xu Zhi-Na, Wang Jun-Xi, Wei Dong-Min, Pan Xin-Liang

机构信息

Department of Otolaryngology-Head and Neck Surgery, Qi-Lu Hospital, Shandong University, Jinan, China.

出版信息

Asian Pac J Cancer Prev. 2012;13(5):2133-7. doi: 10.7314/apjcp.2012.13.5.2133.

DOI:10.7314/apjcp.2012.13.5.2133

PMID:22901182

Abstract

OBJECTIVE

To investigate the prognostic role of antigen KI-67 (Ki-67) and G1/S-specific cyclin-D1 (cyclin-D1) in patients with laryngeal squamous cell carcinoma (LSCC).

METHODS

Immunohistochemical staining (IHS) was used to determine the protein expression of Ki-67 and cyclin-D1 in LSCC tissues. Kaplan-Meier survival curves was calculated with reference to Ki-67 and cyclin-D1 levels.

RESULTS

Cyclin-D1 and Ki67 were expressed in the nuclei of cancer cells. Among the total of 92 cancer tissues examined by immunohistochemistry, 60 (65.22%) had cyclin-D1 overexpression and 56 (60.87%) had Ki67 overexpression. Cyclin-D1 overexpression is associated with the advanced stage of the cancer (P=0.029), but not with gender, age, stage of cancer, histological differentiation, anatomical site, smoking history and alcohol consumption history. Ki67 overexpression is not associated with the advanced stage, gender, age, histological differentiation, anatomical site, smoking history and alcohol consumption history. A statistically significant correlation was found between lymph node status and the expression of Ki67 (p=0.025). Overexpression of cyclin-D1 was correlated to shorter relapse-free survival period (P<0.001).

CONCLUSIONS

Overexpression of cyclin-D1 can be used as a marker to predict relapse in patients with LSCC after primary curative resection.

摘要

目的

探讨抗原KI-67（Ki-67）和G1/S特异性细胞周期蛋白D1（细胞周期蛋白D1）在喉鳞状细胞癌（LSCC）患者中的预后作用。

方法

采用免疫组织化学染色（IHS）法检测LSCC组织中Ki-67和细胞周期蛋白D1的蛋白表达。参照Ki-67和细胞周期蛋白D1水平计算Kaplan-Meier生存曲线。

结果

细胞周期蛋白D1和Ki67在癌细胞核中表达。在通过免疫组织化学检查的92个癌组织中，60个（65.22%）细胞周期蛋白D1过表达，56个（60.87%）Ki67过表达。细胞周期蛋白D1过表达与癌症晚期相关（P=0.029），但与性别、年龄、癌症分期、组织学分化、解剖部位、吸烟史和饮酒史无关。Ki67过表达与晚期、性别、年龄、组织学分化、解剖部位、吸烟史和饮酒史无关。淋巴结状态与Ki67表达之间存在统计学显著相关性（p=0.025）。细胞周期蛋白D1过表达与无复发生存期缩短相关（P<0.001）。