Ahmed Rehab Allah, Shawky Abd El-Aty, Hamed Rasha Hamdy
Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Pathol Oncol Res. 2014 Jul;20(3):625-33. doi: 10.1007/s12253-014-9741-6. Epub 2014 Jan 29.
Cyclin D1 and E-cadherin are important factors in the progression and metastasis of cancers. Their role in laryngeal carcinoma has been studied with conflicting results. To define the frequency of cyclin D1 and E-cadherin expression and its correlation with both the clinicopathological characteristics and prognosis of patients with laryngeal squamous cell carcinoma (LSCC). Tumor tissue samples from 75 patients with laryngeal squamous cell carcinoma were examined for cyclin D1 and E-cadherin expression by immunohistochemistry. The relationship between the expression of both molecules and the age and sex of the patient, tumor site, tumor differentiation, lymph node metastasis, tumor invasiveness, TNM stages, tumor recurrence and overall survival was analyzed. Cyclin D1 was found to be a significant independent prognostic factor of lymph node metastasis (p = 0.000). The multivariate analysis revealed that cyclin D1 and E-cadherin expression wasn't an independent prognostic factor of local recurrence free survival (LRFS) in patients with LSCC (P = 0.56 and 0.28) respectively. However, the univariate analysis revealed a significant association between them and LRFS (p = 0.003 and 0.000) respectively. Also, the group of high cyclin D1 /low E-cadherin expression had the poorest prognosis, so they might serve as potential predictors of the prognosis of the patients with LSCC. E-cadherin was found to affect the overall survival (OAS) significantly by the univariate analysis (p = 0.01). However, by the multivariate analysis the TNM stage was the only independent prognostic factor of OAS (p < 0.05). Cyclin D1 can be used as an independent prognostic marker of lymph node metastasis in patients with LSCC and can help to identify those patients with clinically negative lymph nodes but with considerable risk for occult metastasis. Detection of cyclin D1 and E-cadherin status in LSCC may contribute to the identification of patients with high risk factors of local recurrence. However, they don't appear to be better prognostic predictors than other established markers in LSCC.
细胞周期蛋白D1和E-钙黏蛋白是癌症进展和转移中的重要因素。它们在喉癌中的作用已有研究，但结果相互矛盾。为了确定细胞周期蛋白D1和E-钙黏蛋白表达的频率及其与喉鳞状细胞癌（LSCC）患者临床病理特征和预后的相关性。采用免疫组织化学方法检测75例喉鳞状细胞癌患者肿瘤组织样本中细胞周期蛋白D1和E-钙黏蛋白的表达。分析了这两种分子的表达与患者年龄、性别、肿瘤部位、肿瘤分化、淋巴结转移、肿瘤侵袭性、TNM分期、肿瘤复发和总生存之间的关系。发现细胞周期蛋白D1是淋巴结转移的一个重要独立预后因素（p = 0.000）。多因素分析显示，细胞周期蛋白D1和E-钙黏蛋白表达分别不是LSCC患者无局部复发生存期（LRFS）的独立预后因素（P = 0.56和0.28）。然而，单因素分析分别显示它们与LRFS之间存在显著关联（p = 0.003和0.000）。此外，高细胞周期蛋白D1/低E-钙黏蛋白表达组的预后最差，因此它们可能作为LSCC患者预后的潜在预测指标。单因素分析发现E-钙黏蛋白对总生存期（OAS）有显著影响（p = 0.01）。然而，多因素分析显示TNM分期是OAS的唯一独立预后因素（p < 0.05）。细胞周期蛋白D1可作为LSCC患者淋巴结转移的独立预后标志物，有助于识别那些临床淋巴结阴性但有隐匿转移高风险的患者。检测LSCC中细胞周期蛋白D1和E-钙黏蛋白状态可能有助于识别具有局部复发高危因素的患者。然而，在LSCC中，它们似乎不是比其他已确立的标志物更好的预后预测指标。
