Zhou Yongchun, Chen Yan, Wang Xicai, Liu Xin, Shi Hutao, Yao Qian, Jin Congguo, Wu Zhiping, Huang Yunchao
Yunnan Tumor Research Institute, the Third Affiliated Hospital of Kunming Medical University, Kunming 650118, China.
Zhongguo Fei Ai Za Zhi. 2012 Aug;15(8):449-55. doi: 10.3779/j.issn.1009-3419.2012.08.01.
The incidence of Xuanwei lung cancer ranks first in China, and its pathogenesis requires in-depth investigation. This study aims to establish an orthotopic Xuanwei lung cancer severe combined immunodeficiency (SCID) mouse model and to provide a basic experimental platform for further study.
The Xuanwei lung cancer cell line XWLC-05 was inoculated into the lung tissue of SCID mice in high and low doses. The tumor formation rates, tumor characteristics, spontaneous metastases, and survival times of the mice were observed, taking a subcutaneously transplanted tumor as control.
The tumor formation rates of the orthotopic transplantation of lung cancer cells in high and low doses were 81% and 83%, respectively, among which mice in the high-dose group appeared cachectic on day 13. Extensive invasion and adhesion were observed in the contralateral lung and thoracic cavity, but no distant metastasis was exhibited. Mice with low-dose cells in the orthotopic transplantation group appeared cachectic and distant metastasis occurred on day 25. The tumor formation rates in the subcutaneous inoculation group by the high and low doses of cells were 100% and 94.5%, respectively, and no distant metastasis was observed. The rate of metastasis within the orthotopic transplantation group and between the orthotopic and subcutaneous inoculation groups showed a significant difference (P<0.05). A significant difference was indicated by the survival rate within and between the groups (P<0.001).
We successfully established an orthotopic XWLC SCID mouse model, which lays the foundation for a more in-depth study.
宣威肺癌发病率居中国首位，其发病机制有待深入研究。本研究旨在建立原位宣威肺癌严重联合免疫缺陷（SCID）小鼠模型，为进一步研究提供基础实验平台。
将宣威肺癌细胞系XWLC - 05高、低剂量接种于SCID小鼠肺组织，以皮下移植瘤为对照，观察小鼠肿瘤形成率、肿瘤特征、自发转移情况及生存时间。
高、低剂量肺癌细胞原位移植的肿瘤形成率分别为81%和83%，其中高剂量组小鼠在第13天出现恶病质，对侧肺和胸腔可见广泛侵袭和粘连，但无远处转移。原位移植低剂量细胞组小鼠在第25天出现恶病质并发生远处转移。高、低剂量细胞皮下接种组的肿瘤形成率分别为100%和94.5%，未见远处转移。原位移植组内及原位与皮下接种组之间的转移率差异有统计学意义（P<0.05）。组内及组间生存率差异有统计学意义（P<0.001）。
成功建立了原位XWLC SCID小鼠模型，为更深入研究奠定了基础。
