pJP5603 自杀载体的全序列。
Complete sequence of the suicide vector pJP5603.
机构信息
Helmholtz-Centre for Infection Research, Group Microbial Communication, Braunschweig, Germany.
出版信息
Plasmid. 2013 Jan;69(1):104-7. doi: 10.1016/j.plasmid.2012.07.005. Epub 2012 Aug 9.
Abstract
We have sequenced the complete R6K-based and mobilizable suicide vector pJP5603. For the replication of the vector a trans supply of the pir-encoded π protein of plasmid R6K is essential. The 3.126 kb plasmid encodes a kanamycin resistance cassette for selection and contains a lacZ-α-system that allows a blue-white selection of cloned fragments.
摘要
我们已经对基于 R6K 的可移动自杀载体 pJP5603 进行了测序。该载体的复制需要 R6K 质粒上 pir 编码的 π 蛋白的转供。该 3.126 kb 大小的质粒还编码一个卡那霉素抗性盒用于筛选，同时还包含一个 lacZ-α 系统，可用于克隆片段的蓝白斑选择。
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