Argento Diagnostics Ltd, Knowledge & Innovation Centre, National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, UK.
J Immunol Methods. 2013 Jan 31;387(1-2):303-7. doi: 10.1016/j.jim.2012.08.001. Epub 2012 Aug 8.
The application of silver nanoparticles to electrochemical metalloimmunoassays has previously been reported (Szymanski et al., 2009) and is now used in the electrochemical Argento immunoassay platform via UK Patent No. 2458420. The development of an immunoassay in this format requires the optimisation of the antibody to silver nanoparticle conjugation process. Issues such as pH, antibody concentration and other factors can affect the assay performance. In order to determine the effect of these variables it is necessary to understand and control the effects of other factors that may affect the assay signal. In this study a number of conditions which affect the assay signal were identified and methods developed to minimise variability in the assay signal, resulting in a standardised method allowing easy comparisons of silver nanoparticle conjugates prepared and assayed at different times.
先前已有报道将银纳米粒子应用于电化学免疫测定（Szymanski 等人，2009），现通过英国专利号 2458420 将其应用于电化学 Argento 免疫测定平台。这种格式的免疫测定的发展需要优化抗体与银纳米粒子的偶联过程。pH 值、抗体浓度和其他因素等问题会影响测定性能。为了确定这些变量的影响，有必要了解和控制可能影响测定信号的其他因素的影响。在这项研究中，确定了影响测定信号的一些条件，并开发了一些方法来最小化测定信号的可变性，从而形成一种标准化方法，允许轻松比较在不同时间制备和测定的银纳米粒子偶联物。
