Jiang Hu, Li Xiangmin, Xiong Ying, Pei Ke, Nie Lijuan, Xiong Yonghua
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China.
Toxins (Basel). 2017 Feb 28;9(3):83. doi: 10.3390/toxins9030083.
A silver nanoparticle (AgNP)-based fluorescence-quenching lateral flow immunoassay with competitive format (cLFIA) was developed for sensitive detection of ochratoxin A (OTA) in grape juice and wine samples in the present study. The Ru(phen) 3 2 + -doped silica nanoparticles (RuNPs) were sprayed on the test and control line zones as background fluorescence signals. The AgNPs were designed as the fluorescence quenchers of RuNPs because they can block the exciting light transferring to the RuNP molecules. The proposed method exhibited high sensitivity for OTA detection, with a detection limit of 0.06 µg/L under optimized conditions. The method also exhibited a good linear range for OTA quantitative analysis from 0.08 µg/L to 5.0 µg/L. The reliability of the fluorescence-quenching cLFIA method was evaluated through analysis of the OTA-spiked red grape wine and juice samples. The average recoveries ranged from 88.0% to 110.0% in red grape wine and from 92.0% to 110.0% in grape juice. Meanwhile, less than a 10% coefficient variation indicated an acceptable precision of the cLFIA method. In summary, the new AgNP-based fluorescence-quenching cLFIA is a simple, rapid, sensitive, and accurate method for quantitative detection of OTA in grape juice and wine or other foodstuffs.
本研究开发了一种基于银纳米颗粒（AgNP）的具有竞争模式的荧光猝灭侧向流动免疫分析方法（cLFIA），用于灵敏检测葡萄汁和葡萄酒样品中的赭曲霉毒素A（OTA）。将钌（Ⅱ）-邻菲罗啉掺杂的二氧化硅纳米颗粒（RuNPs）喷涂在测试线和对照线区域作为背景荧光信号。AgNPs被设计为RuNPs的荧光猝灭剂，因为它们可以阻断激发光传递到RuNP分子。所提出的方法对OTA检测具有高灵敏度，在优化条件下检测限为0.06 μg/L。该方法在0.08 μg/L至5.0 μg/L的OTA定量分析中也表现出良好的线性范围。通过分析添加OTA的红葡萄酒和果汁样品评估了荧光猝灭cLFIA方法的可靠性。红葡萄酒中的平均回收率为88.0%至110.0%，葡萄汁中的平均回收率为92.0%至110.0%。同时，小于10%的变异系数表明cLFIA方法具有可接受的精密度。总之，新的基于AgNP的荧光猝灭cLFIA是一种用于定量检测葡萄汁、葡萄酒或其他食品中OTA的简单、快速、灵敏且准确的方法。
