Sakr Saber A, Shalaby Somaya Y
Department of Zoology, Faculty of Science, Menoufiya University, Shebin El-kom, Egypt.
Toxicol Ind Health. 2014 Apr;30(3):259-67. doi: 10.1177/0748233712456059. Epub 2012 Aug 17.
Carbendazim is a broad spectrum carbamate fungicide used in the control of various fungal pathogens. Licorice (Glycyrrhiza glabra) is one of the widely used medicinal plants in oriental nations. The present work studied the effect of licorice aqueous extract on carbendazim-induced testicular toxicity in albino rats. Administration of carbendazim induced significant decrease in testis weight, diameter, and germinal epithelial height of the seminiferous tubules. Histological results revealed degeneration of seminiferous tubules, loss of spermatogenic cells, and apoptosis. Moreover, carbendazim caused elevation of testicular malondialdehyde (MDA), marker of lipid peroxidation, and reduced the activity of the antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT). Coadministration of licorice extract with carbendazim improved the histomorphological and histopathological changes observed in animals treated with carbendazim. In addition, licorice treatment leads to a significant decrease in the level of MDA and increase in the activities of SOD and CAT. According to the present results, it is concluded that licorice aqueous extract can improve the testicular toxicity of carbendazim and this effect may be attributed to antioxidant properties of one or more of its constituents.
多菌灵是一种用于防治多种真菌病原体的广谱氨基甲酸酯类杀菌剂。甘草（Glycyrrhiza glabra）是东方国家广泛使用的药用植物之一。本研究探讨了甘草水提取物对多菌灵诱导的白化大鼠睾丸毒性的影响。多菌灵给药导致睾丸重量、直径和生精小管生精上皮高度显著降低。组织学结果显示生精小管变性、生精细胞丢失和凋亡。此外，多菌灵导致睾丸丙二醛（MDA）水平升高，MDA是脂质过氧化的标志物，并降低了抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。甘草提取物与多菌灵联合给药改善了多菌灵处理动物中观察到的组织形态学和组织病理学变化。此外，甘草处理导致MDA水平显著降低，SOD和CAT活性增加。根据目前的结果，得出结论：甘草水提取物可以改善多菌灵的睾丸毒性，这种作用可能归因于其一种或多种成分的抗氧化特性。
