Department of Biochemistry & Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry 605014, India.
Environ Toxicol Pharmacol. 2013 May;35(3):444-53. doi: 10.1016/j.etap.2013.01.010. Epub 2013 Feb 9.
The study evaluated the protective role of kolaviron (an isolated biflavonoid from the seed of Garcinia kola) and vitamin E in carbendazim-induced reproductive dysfunction in male rats. Adult male Wistar rats were orally exposed to carbendazim (200mg/kg) singly or in combination with kolaviron (100 and 200mg/kg). Exposure to carbendazim significantly decreased the activities of superoxide dismutase and catalase but markedly increased sialic acid concentration and lipid peroxidation in the testes of rats. Western blot analysis revealed that carbendazim treatment decreased the expression of steroid acute regulatory (StAR) protein and androgen binding protein (ABP) with concomitant decrease in activities of steroidogenic enzymes. Germ cell apoptosis in carbendazim-treated rats was confirmed by TUNEL assay. However, pretreatment with kolaviron and vitamin E restored the testicular antioxidant status and steroidogenesis and decreased apoptotic nuclei to near control level in carbendazim-treated rats. Kolaviron may prove useful in combating carbendazim-induced reproductive toxicity.
本研究评估了 kolaviron（从可乐果种子中分离出的双黄酮）和维生素 E 在苯菌灵诱导的雄性大鼠生殖功能障碍中的保护作用。成年雄性 Wistar 大鼠经口暴露于苯菌灵（200mg/kg）单药或与 kolaviron（100 和 200mg/kg）联合暴露。苯菌灵暴露显著降低了睾丸中超氧化物歧化酶和过氧化氢酶的活性，但显著增加了大鼠睾丸中唾液酸的浓度和脂质过氧化。Western blot 分析显示，苯菌灵处理降低了类固醇急性调节蛋白（StAR）蛋白和雄激素结合蛋白（ABP）的表达，并伴有甾体生成酶活性的降低。TUNEL 检测证实了苯菌灵处理大鼠的生殖细胞凋亡。然而，kolaviron 和维生素 E 的预处理恢复了睾丸的抗氧化状态和甾体生成，并将苯菌灵处理大鼠的凋亡核数降低到接近对照水平。Kolaviron 可能有助于对抗苯菌灵诱导的生殖毒性。
