College of Medicine, Nankai University, Tianjin 300071, China.
Arch Toxicol. 2013 Jun;87(6):1067-73. doi: 10.1007/s00204-012-0925-0. Epub 2012 Aug 18.
Nanosized copper oxide (nano-CuO) has been widely used in many fields. Recent studies have shown that nano-CuO has toxic effects on various organs, but the effects of nano-CuO on kidney remain unclear. The aim of this study was to assess whether nano-CuO can induce the apoptosis of podocytes. The result of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay indicated that nano-CuO decreased podocyte viability in a concentration-dependent and time-dependent manner. The apoptotic assay by flow cytometry showed that nano-CuO induced podocyte apoptosis significantly. Meanwhile, the result of flow cytometric assay gave a clear indication that nano-CuO increased reactive oxygen species (ROS) level. The measurement of superoxide dismutase (SOD) and malondialdehyde (MDA) showed that nano-CuO decreased SOD and increased MDA levels in podocytes. These results may provide basic information for the safe application of nano-CuO in the future.
纳米氧化铜（nano-CuO）已被广泛应用于许多领域。最近的研究表明，nano-CuO 对各种器官具有毒性作用，但 nano-CuO 对肾脏的影响尚不清楚。本研究旨在评估 nano-CuO 是否会诱导足细胞凋亡。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）检测结果表明，nano-CuO 以浓度和时间依赖的方式降低足细胞活力。流式细胞术的凋亡检测表明，nano-CuO 显著诱导足细胞凋亡。同时，流式细胞术检测结果清楚地表明，nano-CuO 增加了活性氧（ROS）水平。超氧化物歧化酶（SOD）和丙二醛（MDA）的测定表明，nano-CuO 降低了足细胞中的 SOD 并增加了 MDA 水平。这些结果可能为 nano-CuO 在未来的安全应用提供基础信息。
