Asadzadeh Jamshid, Asadi Malek Hossein, Shakhssalim Nasser, Rafiee Mahmoud-Reza, Kalhor Hamid Reza, Tavallaei Mahmoud, Mowla Seyed Javad
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Urol J. 2012 Summer;9(3):574-80.
To investigate and compare the expression of OCT4B1 between tumor and non-tumor bladder tissues.
We investigated the expression of OCT4B1 in 30 tumor and non-tumor surgical specimens of the bladder, using the TaqMan real-time polymerase chain reaction approach and by carefully designing primers and probes specific for the amplification of the variant.
Most tumor and non-tumor samples of the bladder showed OCT4B1 expression, but its expression level was significantly higher in the tumors (P < .002). Moreover, the up-regulation of OCT4B1 was more significant in high-grade tumors compared to the low-grade ones (P < .05). We have also employed the RNA interference strategy to evaluate the functional role of OCT4B1 in a bladder cancer cell line, 5637. Suppression of OCT4B1 caused some changes in cell cycle distribution, and significantly elevated the rate of apoptosis in the cells.
Our findings suggest that OCT4B1 plays a potential role in tumor initiation and/or progression of the bladder cancer. Additionally, OCT4B1 can be regarded as a new tumor marker for detection, classification, and treatment of the bladder cancer. However, more experimental studies are needed to replicate our findings.
研究并比较OCT4B1在肿瘤性和非肿瘤性膀胱组织中的表达。
我们采用TaqMan实时聚合酶链反应方法，并精心设计用于扩增该变体的引物和探针，研究了30例膀胱肿瘤和非肿瘤手术标本中OCT4B1的表达。
大多数膀胱肿瘤和非肿瘤样本均显示OCT4B1表达，但在肿瘤组织中其表达水平显著更高（P < 0.002）。此外，与低级别肿瘤相比，OCT4B1在高级别肿瘤中的上调更为显著（P < 0.05）。我们还采用RNA干扰策略评估了OCT4B1在膀胱癌细胞系5637中的功能作用。抑制OCT4B1导致细胞周期分布发生一些变化，并显著提高了细胞凋亡率。
我们的研究结果表明，OCT4B1在膀胱癌的肿瘤起始和/或进展中发挥潜在作用。此外，OCT4B1可被视为一种用于膀胱癌检测、分类和治疗的新型肿瘤标志物。然而，需要更多的实验研究来重复我们的发现。
