Molecular Genetics Department, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Cancer Lett. 2011 Oct 28;309(2):170-5. doi: 10.1016/j.canlet.2011.05.027. Epub 2011 Jun 17.
A novel variant of OCT4, OCT4B1, is highly expressed in pluripotent cells. OCT4B1 is also upregulated in various tumors and cell lines. Here, we have constructed a HA-tagged OCT4B1 construct, which could be translated into an N-terminally tagged protein, detectable by HA antibody. Western blotting revealed that OCT4B1 can act as a precursor for OCT4B, and it can also produce a truncated protein with a molecular size corresponding to that of predicted OCT4B1. Furthermore, our data demonstrated a cytoplasmic distribution for both isoforms. Interestingly, the expression ratio of OCT4B1/OCT4B transcripts and proteins was significantly elevated under the heat-stress condition.
一种新型 OCT4 变体 OCT4B1 在多能细胞中高度表达。OCT4B1 在各种肿瘤和细胞系中也上调。在这里，我们构建了一个 HA 标记的 OCT4B1 构建体，它可以翻译成 N 端标记的蛋白质，可被 HA 抗体检测到。Western blot 显示 OCT4B1 可以作为 OCT4B 的前体，它还可以产生与预测的 OCT4B1 相对应的分子大小的截断蛋白。此外，我们的数据表明这两种异构体都存在细胞质分布。有趣的是，在热应激条件下，OCT4B1/OCT4B 转录本和蛋白质的表达比例显著升高。
