Departamento de Química Analítica, Universidade Federal Fluminense, Outeiro de São João Batista s/n, Centro, Niterói, RJ 24020-141, Brazil.
J Fluoresc. 2013 Jan;23(1):49-55. doi: 10.1007/s10895-012-1115-4. Epub 2012 Aug 18.
Statins are a class of drugs mostly used for treating hyperlipidemia, and rosuvastatin is the newest drug in the market belonging to this class. In this present work, a method was developed based on the molecular fluorescence technique, with the objective to quantify rosuvastatin in urine samples. For this purpose, the study of several parameters was made to achieve the maximum analytical signal (under reaction with sulfuric acid during 40 min). Also, a previous step to avoid matrix interference was carried out (liquid-liquid extraction). The limit of detection (LOD) and the limit of quantification (LOQ) were 0.38 and 1.28 mg L(-1), respectively. Linear relationship between rosuvastatin concentration and it's fluorescence intensity was found until 5.0 mg L(-1). The proposed method was tested in several samples spiked with rosuvastatin and recovery was found in the range of 90 ± 10%.
他汀类药物是一类主要用于治疗高脂血症的药物，而罗苏伐他汀是该类药物中最新的市场药物。在本工作中，开发了一种基于分子荧光技术的方法，旨在定量尿液样品中的罗苏伐他汀。为此，进行了多项参数研究，以获得最大的分析信号（在与硫酸反应 40 分钟内）。此外，还进行了前一步骤以避免基质干扰（液-液萃取）。检测限（LOD）和定量限（LOQ）分别为 0.38 和 1.28mg L（-1）。发现罗苏伐他汀浓度与其荧光强度之间存在线性关系，直至 5.0mg L（-1）。该方法在几种添加了罗苏伐他汀的样品中进行了测试，回收率在 90±10%范围内。
