Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Avenida Limeira, 901, 13414-903 Piracicaba, São Paulo, Brazil.
J Dent Res. 2012 Oct;91(10):985-9. doi: 10.1177/0022034512458689. Epub 2012 Aug 17.
Denture liners (DL) are easily colonized by Candida spp. In an attempt to prevent biofilm colonization, manufacturers have incorporated undecylenic acid (UDA) into DL. In this in vitro study, the effects of UDA released from DL on Candida biofilms were investigated. The concentrations of UDA released from commercial DL were determined by gas chromatography-mass spectrometry (GC-MS). Minimum inhibitory concentration (MIC) and minimum fungistatic concentration (MFC) tests were performed for C. albicans or C. glabrata, with UDA for comparison with the concentrations released from DL. Specimens of DL with (experimental group) and without UDA (control group) were fabricated, and Candida biofilms were developed on DL surfaces. Biofilms were evaluated by cell counts, metabolic activity, structure, and secretion of proteinase or phospholipase. The concentrations of UDA released were within the MIC and MFC ranges. In the presence of UDA, C. albicans biofilms were thinner and had lower numbers of viable and active cells, although no significant enzymatic changes were observed relative to the control group (p > 0.05). In contrast, C. glabrata biofilms exhibited higher cell counts and greater metabolic activity and also increased proteinase activity in the presence of UDA relative to the control group (p < 0.05). Overall, UDA did not prevent Candida biofilm formation.
义齿基托（DL）很容易被念珠菌属定植。为了防止生物膜定植，制造商将十一烯酸（UDA）掺入 DL 中。在这项体外研究中，研究了从 DL 释放的 UDA 对念珠菌生物膜的影响。通过气相色谱-质谱联用（GC-MS）测定从商业 DL 释放的 UDA 的浓度。对白色念珠菌或光滑念珠菌进行了 UDA 的最小抑菌浓度（MIC）和最小杀菌浓度（MFC）测试，并与 DL 释放的浓度进行了比较。用含有（实验组）和不含有 UDA（对照组）的 DL 样本制作了 DL，并在 DL 表面上开发了念珠菌生物膜。通过细胞计数、代谢活性、结构以及蛋白酶或磷脂酶的分泌来评估生物膜。释放的 UDA 浓度在 MIC 和 MFC 范围内。在 UDA 的存在下，白色念珠菌生物膜变薄，活细胞和活性细胞数量减少，尽管与对照组相比，酶变化不明显（p > 0.05）。相比之下，与对照组相比，UDA 存在时，光滑念珠菌生物膜的细胞计数更高，代谢活性更强，并且蛋白酶活性也增加（p < 0.05）。总体而言，UDA 不能防止念珠菌生物膜的形成。
