Sapmaz E, Kale A, Akpolat N
Obstetrics and Gynecology Department, Firat University School of Medicine, Elazig, Turkey.
Clin Exp Obstet Gynecol. 2012;39(2):186-90.
To investigate the effects of melatonin use in unilateral total salpingectomy on ovarian histology in rats.
Firat University, Medical School, Obstetrics and Gynecology Department, Elazig.
Thirty adult, female rats of Wistar albino species with regular cycles were randomly allocated to three groups in the estrus phase. G1 (n: 10): The group where the abdomen was opened and closed, and left oophorectomy was performed six months later. G2 (n: 10): The group where left total salpingectomy was performed and followed by left oophorectomy six months later. G3 (n: 10): The group where the abdomen was opened, left total salpingectomy was performed 15 min after 10 mg/kg/IP melatonin administration, and left oophorectomy was performed six months later. Samples of the left ovary were fixed in formaldehyde. The preparations were stained with hematoxylineosin, and primordial, primary, secondary and tertiary follicles were counted. All the numbers were added up to determine the ovarian follicle reserve. Atretic follicles were counted. Corpus luteum and corpus albicans were counted. Number of total corpuses was calculated. Regression of the presence of angiogenesis within the corpus luteum was examined. Presence of fibrosis on the ovarian stroma was examined. An ordinal scale was formed for the presence of regression of angiogenesis within the corpus luteum and presence of fibrosis (none: 0p, present: 1p, markedly present: 2p). Follicle cysts in the ovary were counted. Kruskal Wallis variance analysis was used in the statistical analysis of data; p < 0.05 was considered significant.
Primordial follicle count, ovarian follicle reserve and regression of angiogenesis in the corpus luteum were found to be significantly lower (p < 0.05, Mann-Whitney U test), and atretic follicle count, microscopic follicle cyst and fibrosis development were found to be significantly higher in G2, when compared to G1 and G3 (p < 0.05, Mann-Whitney U test). None of the rats in G1 and G3 had ovarian cysts, whereas five rats in G2 were identified as having macroscopic follicle cysts. Other data were found to be similar in G1, G2, and G3 (Kruskal Wallis variance analysis).
Left total salpingectomy reduces primordial follicles, ovarian follicle reserve and regression of angiogenesis in the corpus luteum, while increasing atretic follicles, microscopic ovarian cysts and fibrosis development. It leads to the development of macroscopic follicle cysts in the ovary at a high rate (50%) in the sixth month. Melatonin use eliminates these harmful effects. Melatonin can be used to avoid the unfavorable effect of total salpingectomy on the ovary.
研究褪黑素对大鼠单侧全输卵管切除术卵巢组织学的影响。
土耳其埃尔津詹市菲拉特大学医学院妇产科。
将30只月经周期规律的成年雌性Wistar白化大鼠在发情期随机分为三组。G1组（n = 10）：打开并关闭腹腔，6个月后行左侧卵巢切除术。G2组（n = 10）：行左侧全输卵管切除术，6个月后行左侧卵巢切除术。G3组（n = 10）：打开腹腔，腹腔注射10mg/kg褪黑素15分钟后行左侧全输卵管切除术，6个月后行左侧卵巢切除术。取左侧卵巢样本用甲醛固定。标本用苏木精-伊红染色，计数原始卵泡、初级卵泡、次级卵泡和三级卵泡。将所有数量相加以确定卵巢卵泡储备。计数闭锁卵泡。计数黄体和白体。计算黄体总数。检查黄体内部血管生成的消退情况。检查卵巢基质的纤维化情况。根据黄体内部血管生成的消退情况和纤维化情况形成一个有序量表（无：0分，存在：1分，明显存在：2分）。计数卵巢中的卵泡囊肿。数据的统计分析采用Kruskal Wallis方差分析；p < 0.05被认为具有统计学意义。
与G1组和G3组相比，G2组的原始卵泡计数、卵巢卵泡储备和黄体血管生成的消退明显降低（p < 0.05，Mann-Whitney U检验），闭锁卵泡计数、微小卵泡囊肿和纤维化发展明显更高（p < 0.05，Mann-Whitney U检验）。G1组和G3组均无大鼠出现卵巢囊肿，而G2组有5只大鼠被确定有肉眼可见的卵泡囊肿。G1、G2和G3组的其他数据相似（Kruskal Wallis方差分析）。
左侧全输卵管切除术会减少原始卵泡、卵巢卵泡储备和黄体血管生成的消退，同时增加闭锁卵泡、微小卵巢囊肿和纤维化发展。在第六个月时，它会导致卵巢中肉眼可见的卵泡囊肿以较高比例（50%）发展。使用褪黑素可消除这些有害影响。褪黑素可用于避免全输卵管切除术对卵巢的不利影响。
