Atilgan Remzi, Kuloğlu Tuncay, Boztosun Abdullah, Orak Uğur, Baspinar Melike, Can Behzat, Sapmaz Ekrem
Firat University School of Medicine, Department of Obstetrics and Gynecology, Elazig, Turkey.
Firat University School of Medicine, Department of Histology, Elazig, Turkey.
Eur J Obstet Gynecol Reprod Biol. 2015 May;188:56-60. doi: 10.1016/j.ejogrb.2015.02.028. Epub 2015 Feb 26.
We aimed to investigate the effects of unilateral total salpingectomy procedure on ovarian follicular reserve, apoptosis, and proliferating cell nuclear antigen (PCNA) staining in this study.
Fourteen female Wistar Albino rats of 12 weeks were randomly divided into two groups. Abdomen midline incision was conducted under general anesthesia. Group 1(G1) (n=7): Group in which only the abdomen was opened and closed, Group 2(G2) (n=7): Group that underwent right total salpingectomy. After 1 month, abdomens of all rats were opened. Ovaries were macroscopically evaluated. Right ovarian tissue was quickly removed, fixed in 10% formaldehyde, and paraffin blocks were prepared.' The existence of fibrosis was identified with the usage of light microscope. Follicles were microscopically classified and counted. The prevalence of cytoplasmic immune staining and TUNEL staining was scored semi-quantitatively.
SPSS 17.0 software was used for the statistical analysis of data. First, Kruskal-Wallis variance analysis was conducted, and then Mann-Whitney U test was utilized for inter-group dual comparisons for parameters found as p<0.05.
While the number of CL was found out dramatically high, secondary follicle count was found out to be significantly low in G2. Also in G2, although the number of atretic follicle and fibrosis were found out significantly increased, and the score of the angiogenesis was found to be significantly decreased in CL. When compared PCNA immunoreactivity in granulosa cells with the control group, there was a significant decrease in G2. When compared the malondialdehyde (MDA) immunoreactivity with G1 a significant increase was established in G2. Apoptosis score of ovarian follicles in granulosa cells was significantly higher in G2.
In this experimental study, the decrease in the ovarian reserve and PCNA staning of granulosa cells, an increase in apoptosis, fibrosis and the number of atretic follicles in unilateral total salpingectomy operation were analyzed in rats. We found out significantly higher MDA staining rates in G2 in comparison to in G1. According to the study, the unilateral total salpingectomy procedure can damage to the same side ovarian tissue by means of the ischemia and reperfusion injury at the ovarian tissue.
本研究旨在探讨单侧全输卵管切除术对卵巢卵泡储备、细胞凋亡及增殖细胞核抗原（PCNA）染色的影响。
将14只12周龄的雌性Wistar白化大鼠随机分为两组。在全身麻醉下进行腹部正中切口。第1组（G1）（n = 7）：仅打开并关闭腹部的组；第2组（G2）（n = 7）：接受右侧全输卵管切除术的组。1个月后，打开所有大鼠的腹部。对卵巢进行宏观评估。迅速取出右侧卵巢组织，用10%甲醛固定，制备石蜡块。使用光学显微镜确定纤维化的存在。对卵泡进行显微镜分类和计数。对细胞质免疫染色和TUNEL染色的发生率进行半定量评分。
采用SPSS 17.0软件对数据进行统计分析。首先进行Kruskal-Wallis方差分析，然后对p<0.05的参数进行组间双比较时采用Mann-Whitney U检验。
G2组中闭锁卵泡（CL）数量显著增多，次级卵泡数量显著减少。同样在G2组中，虽然闭锁卵泡和纤维化数量显著增加，且CL中血管生成评分显著降低。与对照组相比，G2组颗粒细胞中PCNA免疫反应性显著降低。与G1组相比，G2组丙二醛（MDA）免疫反应性显著升高。G2组颗粒细胞中卵巢卵泡的凋亡评分显著更高。
在本实验研究中，分析了大鼠单侧全输卵管切除术中卵巢储备和颗粒细胞PCNA染色的降低、细胞凋亡、纤维化及闭锁卵泡数量的增加。我们发现G2组的MDA染色率显著高于G1组。根据该研究，单侧全输卵管切除术可通过卵巢组织的缺血再灌注损伤对同侧卵巢组织造成损害。
