Şimşek Mehmet, Kuloğlu Tuncay, Pala Şehmus, Boztosun Abdullah, Can Behzat, Atilgan Remzi
Department of Obstetrics and Gynecology, Firat University School of Medicine, Elazig, Turkey.
Department of Histology, Firat University School of Medicine, Elazig, Turkey.
Drug Des Devel Ther. 2015 Mar 5;9:1341-7. doi: 10.2147/DDDT.S76835. eCollection 2015.
To examine the effect of 95% ethanol sclerotherapy (EST) administered over 5 minutes on cyst diameter and ovarian tissue in experimentally induced simple ovarian cysts in a rat model.
In order to induce ovarian cysts, unilateral total salpingectomy was performed in regularly menstruating adult female Wistar albino rats (n=20) between 12 and 14 weeks of age and weighing between 200 and 220 g. One month after the procedure, the abdominal cavity was opened and 14 rats (70%) were found to have developed macroscopic cysts. Rats with macroscopic cysts (n=14) were assigned into two groups in a prospective and single-blinded manner: group 1 (G1) (n=7), control rats; and group 2 (G2) (n=7), 5-minute EST 95% group. Cyst diameter was measured and recorded for each rat. In G2, after whole cyst fluid was aspirated the cystic cavity was irrigated with 95% ethanol, approximately equal to half of the aspirated cyst volume, after which an interval of 5 minutes was allowed and same amount was re-aspirated and the abdominal cavity was closed. One month after this procedure, abdominal cavities were reopened and intra-abdominal adhesion scoring was performed in both groups. Cyst diameter was measured for each rat, and the right ovary was removed, fixed in 10% formaldehyde, and transported to the laboratory. A histologic assessment of the ovarian tissues was performed under light microscopy following staining with hematoxylin and eosin. Mann-Whitney U-test was used for statistical analysis. A P-level less than 0.05 was considered significant.
In comparison with G1, there was a statistically significant reduction in the mean ovarian cyst dimensions in G2, while there were no significant differences between the two groups with respect to total number of follicles. Again, a significant increase in apoptotic activity and germinal epithelial degeneration was observed in G2 as compared to G1. The two groups were similar in terms of adhesion formation.
Although 95% EST results in a reduction in the size of simple ovarian cysts, this effect seems to be achieved at the expense of ovarian tissue injury.
研究在大鼠模型中，5分钟内给予95%乙醇硬化疗法（EST）对实验诱导的单纯性卵巢囊肿直径及卵巢组织的影响。
为诱导卵巢囊肿，对12至14周龄、体重200至220克、月经周期规律的成年雌性Wistar白化大鼠（n = 20）进行单侧全输卵管切除术。术后1个月，打开腹腔，发现14只大鼠（70%）形成了肉眼可见的囊肿。将有肉眼可见囊肿的大鼠（n = 14）以前瞻性单盲方式分为两组：第1组（G1）（n = 7），为对照大鼠；第2组（G2）（n = 7），即5分钟95%乙醇EST组。测量并记录每只大鼠的囊肿直径。在G2组中，吸出全部囊液后，用约等于吸出囊液体积一半的95%乙醇冲洗囊腔，5分钟后再次吸出等量液体，然后关闭腹腔。此操作1个月后，再次打开腹腔，对两组进行腹腔内粘连评分。测量每只大鼠的囊肿直径，切除右侧卵巢，固定于10%甲醛中，送至实验室。苏木精-伊红染色后，在光学显微镜下对卵巢组织进行组织学评估。采用曼-惠特尼U检验进行统计分析。P值小于0.05被认为具有统计学意义。
与G1组相比，G2组卵巢囊肿平均尺寸有统计学显著减小，而两组卵泡总数无显著差异。此外，与G1组相比，G2组凋亡活性和生发上皮变性显著增加。两组在粘连形成方面相似。
尽管95%乙醇EST可使单纯性卵巢囊肿尺寸减小，但这种效果似乎是以卵巢组织损伤为代价实现的。
