Hornig Nora, Färber-Schwarz Aline
Institute of Cell Biology and Immunology, University of Stuttgart, Stuttgart, Germany.
Methods Mol Biol. 2012;907:713-27. doi: 10.1007/978-1-61779-974-7_40.
Recombinant bispecific antibodies have many different applications; especially promising is their therapeutic potential due to their ability to retarget an effector molecule or a cell to a disease-related target structure. In the last years, many formats have been developed: two commonly used are the bispecific diabody and the tandem scFv. In this chapter, the cloning, bacterial production, purification, and characterization of the two antibody formats are described in detail.
重组双特异性抗体有许多不同的应用；因其能够将效应分子或细胞重新靶向与疾病相关的靶结构，所以其治疗潜力尤其令人期待。在过去几年中，已开发出多种形式：两种常用的是双特异性双抗体和串联单链抗体片段。在本章中，将详细描述这两种抗体形式的克隆、细菌生产、纯化及特性分析。
