McGuinness B T, Walter G, FitzGerald K, Schuler P, Mahoney W, Duncan A R, Hoogenboom H R
Cambridge Antibody Technology Ltd., Melbourn, Cambridgeshire, UK.
Nat Biotechnol. 1996 Sep;14(9):1149-54. doi: 10.1038/nbt0996-1149.
Methods for the generation of large numbers of different bispecific antibodies are presented. Cloning strategies are detailed to create repertoires of bispecific diabody molecules with variability on one or both of the antigen binding sites. This diabody format, when combined with the power of phage display technology, allows the generation and analysis of thousands of different bispecific molecules. Selection for binding presumably also selects for more stable diabodies. Phage diabody libraries enable screening or selection of the best combination bispecific molecule with regards to affinity of binding, epitope recognition and pairing before manufacture of the best candidate.
本文介绍了产生大量不同双特异性抗体的方法。详细阐述了克隆策略，以创建在一个或两个抗原结合位点具有变异性的双特异性双体分子文库。这种双体形式与噬菌体展示技术相结合，能够产生和分析数千种不同的双特异性分子。对结合的筛选可能也会选择更稳定的双体。噬菌体双体文库能够在制造最佳候选物之前，就结合亲和力、表位识别和配对情况，筛选或选择出最佳组合的双特异性分子。
