通过种间杂交对磷酸乙醇酸磷酸酶（PGP）基因在16号染色体上的定位（作者译）

[Localization of the gene for phosphoglycolate phosphatase (PGP) on the chromosome 16 by interspecific hybridization (author's transl)].

作者信息

Weil D, Van Cong N, Finaz C, Gross M S, Cochet C, de Grouchy J, Frézal J

出版信息

Hum Genet. 1979 Oct 1;51(2):139-45. doi: 10.1007/BF00287167.

Abstract

Eight primary man-mouse (C11D/TK-) hybrids, twenty three primary and seven secondary man-hamster (CH/HGPRT-) were analyzed for human phosphoglycolate phosphatase (PGP) and for human chromosomes. The following results were obtained: 1. A positive correlation is observed between the chromosome 16 and PGP. 15 hybrids are chr.16+PGP+, 14 hybrids are chr.16-PGP- and 4 hybrids are chr.16-PGP+. 2. The percentage of dissociation between PGP and the chr.16 is low (12%) in comparison with the high percentage of dissociation between PGP and the other autosomes (between 37% and 65%). 3. Excepted the chromosome 16, the other autosomes are observed in hybrids PGP-. These different results indicate the localization of the gene for human PGP on the chromosome 16. The dissociation results chr.16-PGP+ are explained by the breakage of the chr.16 in the hybrids.

摘要

对8个原代人-小鼠（C11D/TK-）杂种细胞、23个原代和7个二代人-仓鼠（CH/HGPRT-）杂种细胞进行了人磷酸乙醇酸磷酸酶（PGP）和人类染色体分析。得到以下结果：1. 观察到16号染色体与PGP之间呈正相关。15个杂种细胞为chr.16+PGP+，14个杂种细胞为chr.16-PGP-，4个杂种细胞为chr.16-PGP+。2. 与PGP和其他常染色体之间的高解离百分比（37%至65%）相比，PGP与chr.16之间的解离百分比很低（12%）。3. 除16号染色体外，在PGP-的杂种细胞中观察到其他常染色体。这些不同结果表明人PGP基因定位于16号染色体上。chr.16-PGP+的解离结果是由杂种细胞中chr.16的断裂所解释的。