Weil D, Van Cong N, Gross M S, Foubert C, Frézal J
Ann Genet. 1980;23(3):150-4.
The value of man-rodent hybrids for detecting creatine kinase BB (CKBB) was greatly increased by the use of an agar technique, in extended incubation (20 hours) with a concentrated solution of creatine as substrate (8 mg/ml instead of 1 mg/ml as usual). The selection of a man-rodent hybrid without discordant between chr. 14 and nucleoside phosphorylase (NP) (a marker of this chromosome) contributed efficiently to the detection of the positive correlation between CKBB and chr. 14/NP. Among 22 independent hybrids, the following were observed : 8 NP + CKBB+, 3 NP + CKBB(+), 11 NP-CKBB- and 6 chr. 14 + CKBB+, 2 chr. 14 + CKBB(+), 11 chr. 14 CKBB-, 2 chr. 14/CKBB+ and 1 chr. 14/CKBB(+). The presence of the chromosome was noted + or - according to its presence in more or less than 30% of the cells. These results show that the gene for CKBB is located on chr. 14 and that the presence of this single chr. 14 is sufficient for the expression of human CKBB in man-rodent hybrids. Discordant results reported by others (Povey et al., 1979) may be due to the weakness of the human CKBB detection in man-rodent hybrids or to the bad conservation of this enzyme.
通过使用琼脂技术，在以浓缩肌酸溶液（8毫克/毫升，而非通常的1毫克/毫升）为底物的延长孵育（20小时）条件下，人-啮齿动物杂种用于检测肌酸激酶BB（CKBB）的价值大幅提高。选择14号染色体与核苷磷酸化酶（NP）（该染色体的一个标记）之间无不一致性的人-啮齿动物杂种，有效地促进了CKBB与14号染色体/NP之间正相关关系的检测。在22个独立的杂种中，观察到以下情况：8个NP + CKBB+、3个NP + CKBB(+)、11个NP-CKBB-以及6个14号染色体 + CKBB+、2个14号染色体 + CKBB(+)、11个14号染色体 CKBB-、2个14号染色体/CKBB+和1个14号染色体/CKBB(+)。根据染色体在超过或少于30%的细胞中的存在情况，标记其存在为 + 或 -。这些结果表明，CKBB基因位于14号染色体上，并且单个14号染色体的存在足以使人-啮齿动物杂种中表达人CKBB。其他人（Povey等人，1979年）报告的不一致结果可能是由于人-啮齿动物杂种中人类CKBB检测的不足，或者是由于该酶保存不佳所致。
