Wittwer-Backofen Ursula
Anthropologie, Medizische Fakultat der Albert Ludwigs, Universitat Frieberg, Freiburg, Germany.
Methods Mol Biol. 2012;915:129-43. doi: 10.1007/978-1-61779-977-8_8.
In Forensic Anthropology age diagnosis of unidentified bodies significantly helps in the identification process. Among the set of established aging methods in anthropology tooth cementum annulation (TCA) is increasingly used due to its narrow error range which can reach 5 years of age in adult individuals at best. The rhythm of cementum appositions of seasonally different density provides a principal mechanism on which TCA is based. Using histological preparation techniques for hard tissues, transversal tooth root sections are produced which can be analyzed in transmitted light microscopy. Even though no standard TCA preparation protocol exists, several methodological validation studies recommend specific treatments depending on individual conditions of the teeth. Individual age is estimated by adding mean tooth eruption age to the number of microscopically detected dark layers which are separated by bright layers and stand for 1 year of age each. To assure a high reliability of the method, TCA age diagnosis has to be based on several teeth of one individual if possible and needs to be supported by different techniques in forensic cases.
在法医人类学中，对身份不明尸体的年龄诊断对身份鉴定过程有显著帮助。在人类学中一系列既定的老化方法中，牙骨质年轮测定法（TCA）因其误差范围窄而越来越多地被使用，在成年人中误差范围最多可达5岁。季节性不同密度的牙骨质沉积节奏是TCA所基于的主要机制。使用硬组织的组织学制备技术制作横向牙根切片，可在透射光显微镜下进行分析。尽管不存在标准的TCA制备方案，但一些方法学验证研究根据牙齿的个体情况推荐了特定的处理方法。通过将平均牙齿萌出年龄加上显微镜下检测到的由明亮层分隔的暗层数量来估计个体年龄，每个暗层代表1岁。为确保该方法的高可靠性，TCA年龄诊断如有可能应基于个体的多颗牙齿，并且在法医案件中需要得到不同技术的支持。
