Department of Socio-Cultural Environmental Studies, The University of Tokyo, Kashiwa, Chiba, Japan.
Water Sci Technol. 2012;66(8):1634-40. doi: 10.2166/wst.2012.360.
Relationships of bacterial populations and extracellular polymer substances (EPS) to dewaterability of activated sludge were studied on three laboratory-scale activated sludge reactors fed with synthetic wastewater. Dewaterability of activated sludge was evaluated by a novel method developed by the authors, in which small amount of sludge was centrifugally dewatered, and its water content was measured. Bacterial populations during the reactor operation were analyzed by the polymerase chain reaction/terminal-restriction fragment length polymorphism (PCR/T-RFLP) targeted at a partial 16S rRNA gene. Extracellular polymeric substances (EPS) were extracted using cation exchange resin (CER), and polysaccharides and total protein in EPS were determined. Some of the dominant terminal-restriction fragments (T-RFs) were observed to have significant relationships with dewaterability of sludge, and it was suggested that bacterial species corresponding to those peaks significantly affected dewaterability. On the other hand, significant relationships were not found between EPS concentration and dewaterability of sludge.
研究了在三个实验室规模的活性污泥反应器中，以合成废水为食时，细菌种群和胞外聚合物物质（EPS）与活性污泥脱水性能的关系。通过作者开发的一种新方法评估了活性污泥的脱水性能，其中少量污泥进行离心脱水，并测量其含水量。通过聚合酶链反应/末端限制性片段长度多态性（PCR/T-RFLP）分析了反应器运行过程中的细菌种群，该方法针对部分 16S rRNA 基因。使用阳离子交换树脂（CER）提取胞外聚合物物质（EPS），并测定 EPS 中的多糖和总蛋白。观察到一些优势末端限制性片段（T-RFs）与污泥的脱水性能有显著关系，这表明与这些峰相对应的细菌种类对脱水性能有显著影响。另一方面，EPS 浓度与污泥的脱水性能之间没有发现显著关系。
