Bukowski L, Erickson K, Lyerla T A
Department of Chemistry, Clark University, Worcester, Massachusetts 01610.
Pigment Cell Res. 1990 Sep;3(3):123-5. doi: 10.1111/j.1600-0749.1990.tb00275.x.
The yellow pigment observed in older axanthic (ax/ax) mutant Mexican axolotls (Ambystoma mexicanum) was analyzed by thin layer chromatography and by spectrofluorometry of its acetyl derivative. Ethanol extracts from the skin of axanthic animals were acetylated and the chloroform-soluble portion of the product mixture was compared with a chloroform solution of an authentic riboflavin tetraacetate standard prepared in the same manner. The pigment in these two solutions behaved identically on thin layer chromatograms and in fluorescent emission spectroscopy. This confirms that the yellow pigment seen in these genetically axanthic animals is riboflavin and, since it cannot be synthesized by the animal, must be derived from the diet.
通过薄层色谱法及其乙酰衍生物的荧光分光光度法，对老龄黄化（ax/ax）突变型墨西哥钝口螈（美西钝口螈）中观察到的黄色色素进行了分析。将黄化动物皮肤的乙醇提取物进行乙酰化处理，并将产物混合物的氯仿可溶部分与以相同方式制备的 authentic 核黄素四乙酸标准品的氯仿溶液进行比较。这两种溶液中的色素在薄层色谱图和荧光发射光谱中的行为相同。这证实了在这些基因黄化动物中看到的黄色色素是核黄素，并且由于动物无法合成它，所以它一定来自饮食。 （注：原文中“authentic”直译为“真实的、可信的、正宗的”等，在这里结合语境意译为“标准的”更合适，但按照要求不添加解释，所以保留原文表述。）
