Armstrong J B
Can J Genet Cytol. 1984 Feb;26(1):1-6. doi: 10.1139/g84-001.
In the Mexican axolotl, Ambystoma mexicanum, gynogenetic diploids can be produced by suppressing the release of the second polar body in eggs activated with irradiated sperm. If the female is heterozygous for a particular mutation, some of the progeny will be homozygous for the mutation. The proportion depends on the distance from the centromere and can be used to determine the gene--centromere (or gene-kinetochore) distance. The mapping function is based on the Neurospora tetrad mapping function. Several variations on this function, based on considerations of how coincidence varies with map distance, are considered. Three genes have been mapped: c at 5.9, t at 24.3, and m at 59.1 map units from their respective centromeres. Four other genes (a, ax, p, and the sex locus) appear to be distant from their centromeres but precise map distances cannot be determined. Based on these data, the total length of the genome has been estimated as at least 2600 map units.
在墨西哥钝口螈（Ambystoma mexicanum）中，通过抑制经辐射精子激活的卵中第二极体的释放，可以产生雌核发育二倍体。如果雌性对于特定突变是杂合的，那么一些后代将对该突变是纯合的。其比例取决于与着丝粒的距离，并且可用于确定基因与着丝粒（或基因与动粒）的距离。该定位函数基于粗糙脉孢菌四分子定位函数。基于对符合度如何随图谱距离变化的考虑，对该函数的几种变体进行了探讨。已定位了三个基因：c距离其各自的着丝粒5.9个图谱单位，t距离24.3个图谱单位，m距离59.1个图谱单位。其他四个基因（a、ax、p和性位点）似乎距离它们的着丝粒较远，但无法确定精确的图谱距离。基于这些数据，基因组的总长度估计至少为2600个图谱单位。
