Biomedical NMR, Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands.
Magn Reson Med. 2013 Jun;69(6):1735-44. doi: 10.1002/mrm.24424. Epub 2012 Aug 20.
In this article, we present a first-pass perfusion imaging protocol to determine quantitative regional perfusion values (in mL min(-1) g(-1)) of the mouse myocardium. Perfusion was quantified using a Fermi-constrained deconvolution of the myocardial tissue response with the arterial input function. A dual-bolus approach was implemented. Experimental evidence is presented for the linearity of signal intensity in the left-ventricular lumen during the prebolus (r=0.99, P<0.001) and in the myocardium during the full-bolus injection (r=0.99, P<0.01) as function of Gd(DTPA)2- injection concentration used. The prebolus was used to reconstruct a nonsaturated arterial input function. Regional perfusion values proved repeatable in a cohort of nine healthy C57BL/6 mice. The perfusion values over two measurements with a 1-week interval were 7.3±0.9 and 7.2±0.6 mL min(-1) g(-1), respectively. No effects of time (P>0.05) and myocardial region (P>0.05) were observed. The between-session coefficient of variation was only 6%, whereas the inter-animal coefficient of variation was 11 and 8% for the separate experiments. We expect that the first-pass perfusion method here presented will be useful in preclinical studies of myocardial perfusion deficits and valuable to assess the impact of pro-angiogenic therapy after myocardial infarction.
在本文中，我们提出了一种首过灌注成像方案，用于确定小鼠心肌的定量区域灌注值（以 mL·min(-1)·g(-1)表示）。灌注通过使用费米约束反卷积对心肌组织响应与动脉输入函数进行量化。采用双脉冲方法。实验证据表明，在预脉冲期间（r=0.99，P<0.001）和在整个脉冲注射期间（r=0.99，P<0.01），左心室腔中的信号强度与使用的 Gd（DTPA）2-注射浓度呈线性关系。预脉冲用于重建不饱和的动脉输入函数。在 9 只健康 C57BL/6 小鼠的队列中，区域灌注值被证明是可重复的。间隔一周进行两次测量的灌注值分别为 7.3±0.9 和 7.2±0.6 mL·min(-1)·g(-1)。未观察到时间（P>0.05）和心肌区域（P>0.05）的影响。两次测量之间的变异系数仅为 6%，而在单独的实验中，动物之间的变异系数分别为 11%和 8%。我们期望这里提出的首过灌注方法将在心肌灌注不足的临床前研究中有用，并有助于评估心肌梗死后促血管生成治疗的影响。
