紫堇灵，一种来自紫堇（紫堇科）的新生物碱及其胆碱酯酶活性。

Corylucinine, a new alkaloid from Corydalis cava (Fumariaceae), and its cholinesterase activity.

作者信息

Novák Zdenĕk, Chlebek Jakub, Opletal Lubomír, Jiros Pavel, Macáková Katerina, Kunes Jiri, Cahlíková Lucie

机构信息

Department of Inorganic and Organic Chemistry, Faculty of Pharmacy, Charles University, , 500 05 Hradec Králové, Czech Republic.

出版信息

Nat Prod Commun. 2012 Jul;7(7):859-60.

PMID:22908564

Abstract

A new benzyldihydroisoquinoline alkaloid (1) was isolated from the tubers of Corydalis cava and named corylucinine. Additionally, 8-trichloromethyl-7,8-dihydropalmatine (2), an isolation artifact of tetrahydropalmatine, was obtained. The structures were established by spectroscopic (including 2D NMR and optical rotation) and HR-ESI-MS methods. Both compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibitory activity. In comparison with the used standards, both compounds showed only moderate inhibitory activity against HuAChE (IC50,. HuAChE = 127.6 +/- 5.2 microM for 1, and IC50, HuAChE = 82.9 +/- 3.9 microM for 2) and none against HuBuChE.

摘要

从延胡索块茎中分离出一种新的苄基二氢异喹啉生物碱（1），命名为紫堇芦碱。此外，还得到了四氢巴马汀的分离假象8 - 三氯甲基 - 7,8 - 二氢巴马汀（2）。通过光谱法（包括二维核磁共振和旋光）和高分辨电喷雾电离质谱法确定了其结构。对这两种化合物进行了人血乙酰胆碱酯酶（HuAChE）和人血浆丁酰胆碱酯酶（HuBuChE）抑制活性测试。与所用标准品相比，这两种化合物对HuAChE仅表现出中等抑制活性（1的IC50，HuAChE = 127.6 +/- 5.2 microM，2的IC50，HuAChE = 82.9 +/- 3.9 microM），对HuBuChE均无抑制活性。