UPLC-MS/MS 分析谱图：来自 科和 科药用植物的具有细胞毒性的生物碱。
UPLC-MS/MS Profile of Alkaloids with Cytotoxic Properties of Selected Medicinal Plants of the and Families.
机构信息
Chair and Department of Pharmaceutical Botany, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland.
Institute of Soil Science and Plant Cultivation, 8 Czartoryskich, 24-100 Puławy, Poland.
出版信息
Oxid Med Cell Longev. 2017;2017:9369872. doi: 10.1155/2017/9369872. Epub 2017 Aug 30.
Cancer is one of the most occurring diseases in developed and developing countries. Plant-based compounds are still researched for their anticancer activity and for their quantity in plants. Therefore, the modern chromatographic methods are applied to quantify them in plants, for example, UPLC-MS/MS (ultraperformance liquid chromatography tandem mass spectrometry). Therefore, the aim of the present study was to evaluate the content of sanguinarine, berberine, protopine, and chelidonine in (L.) Lem., L., Crantz, L., DC., (L.) Vig., (Pursh) Nutt., Willd., and L. For the first time, N,N-dimethyl-hernovine was identified in , , , , , and ; methyl-hernovine was identified in ; columbamine was identified in ; and methyl-corypalmine, chelidonine, and sanguinarine were identified in L. The richest source of protopine among all the examined species was (5463.64 ± 26.3 g/g). The highest amounts of chelidonine and sanguinarine were found in (51,040.0 ± 1.8 g/g and 7925.8 ± 3.3 g/g, resp.), while contained the highest amount of berberine (6358.4 ± 4.2 g/g).
癌症是发达国家和发展中国家最常见的疾病之一。植物化合物仍然因其抗癌活性和在植物中的含量而被研究。因此，现代色谱方法被应用于植物中它们的定量分析，例如 UPLC-MS/MS（超高效液相色谱串联质谱）。因此，本研究的目的是评估在 （L.） Lem.、L.、Crantz、L.、DC.、（L.） Vig.、（Pursh） Nutt.、Willd. 和 L. 中血根碱、小檗碱、原阿片碱和白屈菜碱的含量。首次在 、 、 、 、 和 中鉴定出 N,N-二甲基血根碱；在 中鉴定出甲基血根碱；在 中鉴定出哥伦比亚胺；在 L. 中鉴定出甲基科里棕榈碱、白屈菜碱和血根碱。在所有被检测的物种中，原阿片碱含量最丰富的是 （5463.64±26.3g/g）。在 （51040.0±1.8g/g 和 7925.8±3.3g/g，分别）中发现了最高量的白屈菜碱和血根碱，而 （6358.4±4.2g/g）中含有最高量的小檗碱。
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