Department of Radiotherapy and Radiation Oncology, University of Rostock, Rostock, Germany.
Cancer Invest. 2012 Oct;30(8):593-603. doi: 10.3109/07357907.2012.716469. Epub 2012 Aug 21.
In recent studies, epothilone B was shown to have a cytotoxic and radiosensitizing effect on cells. The aim of our investigation was to explain this impact by examining the mode of action of epothilone B on FaDu and A549 tumor cells. Flow cytometry was used for cell cycle distribution and for the evaluation of apoptosis. Metabolic activity was studied by proliferation assay. Influence on nuclei morphology was investigated by DNA-staining. We showed that epothilone B-induced G2/M accumulation is the main rationale for drug-induced radiosensitivity. The cytotoxic effect resulted in apoptotic cell death, decreased metabolic activity, and formation of multinucleated cells.
在最近的研究中，埃坡霉素 B 被证明对细胞具有细胞毒性和放射增敏作用。我们的研究目的是通过研究埃坡霉素 B 对 FaDu 和 A549 肿瘤细胞的作用机制来解释这种影响。流式细胞术用于细胞周期分布和细胞凋亡的评估。通过增殖测定研究代谢活性。通过 DNA 染色研究对核形态的影响。我们表明，埃坡霉素 B 诱导的 G2/M 期积累是药物诱导放射敏感性的主要原因。细胞毒性作用导致细胞凋亡、代谢活性降低和多核细胞形成。
