Baumgart Tonja, Kriesen Stephan, Neels Oliver, Hildebrandt Guido, Manda Katrin
1Department of Radiotherapy and Radiation Oncology, University Medical Centre Rostock , Rostock , Germany.
Cancer Invest. 2015 Jul;33(6):213-24. doi: 10.3109/07357907.2015.1020115. Epub 2015 Apr 28.
Epothilone B was shown to have promising chemo- and radiosensitizing effects on cells, but the mechanisms underlying cell death remain ambiguous. The aim of the study was to examine selected cell death pathways on the basis of FaDu and A549 cells. Western blot analyses were used for investigation of specific apoptotic markers. Immunofluorescence imaging and flow cytometry were utilized for examination of cell death mechanisms. DNA-staining was used for studying influence of epothilone B on micronucleus rate. We showed that epothilone B can initiate cell death via apoptosis and mitotic catastrophe, but induction of cell death was cell type specific.
埃坡霉素B已被证明对细胞具有有前景的化疗和放射增敏作用，但细胞死亡的潜在机制仍不明确。本研究的目的是基于FaDu细胞和A549细胞来检测特定的细胞死亡途径。采用蛋白质免疫印迹分析来研究特定的凋亡标志物。利用免疫荧光成像和流式细胞术来检测细胞死亡机制。采用DNA染色来研究埃坡霉素B对微核率的影响。我们发现，埃坡霉素B可通过凋亡和有丝分裂灾难引发细胞死亡，但细胞死亡的诱导具有细胞类型特异性。
