体外评价莲心蕉乙醇提取物通过细胞凋亡诱导非激素依赖性人乳腺癌 MDA-MB-231 细胞死亡。
In vitro evaluation of Pandanus amaryllifolius ethanol extract for induction of cell death on non-hormone dependent human breast adenocarcinoma MDA-MB-231 cell via apoptosis.
机构信息
Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
出版信息
BMC Complement Altern Med. 2012 Aug 22;12:134. doi: 10.1186/1472-6882-12-134.
BACKGROUND
Our previous study had shown that P. amaryllifolius was able to selectively inhibit cell proliferation of hormone independent breast cancer cell line MDA-MB-231. To understand the mode of killing and mechanism of action for P. amaryllifolius, the ethanol extract was evaluated for their alteration of cell cycle progression, PS externalization, DNA fragmentation and expression of anti/pro-apoptotic related protein.
RESULTS
Cell cycle progression analysis, Annexin V and Tunel assays suggested that IC50 of P. amaryllifolius ethanol extract induced G0/G1 cell cycle arrest, PS externalization and DNA fragmentation. On the other hand, ELISA for cytochrome c, caspase-3/7, 8 and 9 indicated that apoptosis was contributed by mitochondrial cytochrome c release via induction of caspase 3/7, 9, and p53 was associated with the suppression of XIAP in P. amaryllifolius treated MDA-MB-231 cells.
CONCLUSION
Our findings suggest that P. amaryllifolius ethanol extract induced apoptosis on hormone independent breast cancer cell line MDA-MB-231.
背景
我们之前的研究表明，石蒜能选择性抑制激素非依赖性乳腺癌细胞系 MDA-MB-231 的细胞增殖。为了了解石蒜的杀伤模式和作用机制，我们评估了乙醇提取物对细胞周期进程、PS 外翻、DNA 片段化和抗/促凋亡相关蛋白表达的影响。
结果
细胞周期进展分析、Annexin V 和 Tunel 检测表明，石蒜乙醇提取物的 IC50 诱导 G0/G1 细胞周期阻滞、PS 外翻和 DNA 片段化。另一方面，细胞色素 c、caspase-3/7、8 和 9 的 ELISA 表明，线粒体细胞色素 c 通过诱导 caspase 3/7、9 的释放引起凋亡，p53 与石蒜处理的 MDA-MB-231 细胞中 XIAP 的抑制有关。
结论
我们的研究结果表明，石蒜乙醇提取物诱导激素非依赖性乳腺癌细胞系 MDA-MB-231 发生凋亡。
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