Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland.
Clin Microbiol Infect. 2012 Oct;18(10):E377-9. doi: 10.1111/j.1469-0691.2012.04000.x. Epub 2012 Aug 22.
Bordetella pertussis strains with the pertussis toxin promoter allele ptxP3 have expanded and replaced resident ptxP1 strains in several European countries. We developed an allele-specific real-time PCR method to identify strains with the allele ptxP3, and investigated the emergence of ptxP3 strains by genotyping Finnish clinical isolates (n = 524) from 1953 to 2010. The first ptxP3 strain was detected in 1994, and has become predominant since 2003. Our results demonstrate that the allele-specific real-time PCR is a suitable method for rapid detection of ptxP3 strains, and show the emergence and establishment of ptxP3 strains in Finland.
百日咳博德特氏菌菌株中，带有百日咳毒素启动子等位基因 ptxP3 的菌株已经在几个欧洲国家中扩张并取代了常驻的 ptxP1 菌株。我们开发了一种等位基因特异性实时 PCR 方法来鉴定带有等位基因 ptxP3 的菌株，并通过基因分型研究了 1953 年至 2010 年期间芬兰临床分离株（n = 524）中 ptxP3 菌株的出现情况。第一株 ptxP3 菌株于 1994 年被检测到，自 2003 年以来已成为主要菌株。我们的研究结果表明，等位基因特异性实时 PCR 是一种快速检测 ptxP3 菌株的合适方法，并显示了 ptxP3 菌株在芬兰的出现和确立。
