La Via M F, Self S E
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston.
Recenti Prog Med. 1990 Oct;81(10):629-34.
Flow cytometric cell analysis with fluorescence-labeled antibodies has become a very useful methodology for the immune phenotyping of lymphocytes. The continued evaluation of lymphocyte cell surface antigens has been of value in this respect by providing a clear picture of lymphocyte differentiation steps. Thus it is now possible to precisely identify lymphocytes of abnormal phenotype which may represent malignant cells. Detection of monotypic populations of lymphocytes represents a monoclonal expansion of a lymphocyte subset which is the hallmark of malignancy. In the case of B cell lymphoma, detection of monotypic populations rests on the finding of a monoclonal expansion of a cell type bearing one type of light chain and of heavy chain and/or one of the specific B lymphocyte differentiation antigens. The diagnosis of T cell malignancy is more difficult to establish and a diagnosis of T cell lymphoma rests on the finding of an abnormal phenotype. Thus flow cytometry in combination with histomorphologic examination is a useful technique for the more precise diagnosis of lymphomas and for the establishment of treatment protocols.
使用荧光标记抗体的流式细胞术细胞分析已成为淋巴细胞免疫表型分析非常有用的方法。在这方面，对淋巴细胞细胞表面抗原的持续评估通过提供淋巴细胞分化步骤的清晰图景而具有价值。因此，现在有可能精确识别可能代表恶性细胞的异常表型淋巴细胞。检测淋巴细胞的单克隆群体代表淋巴细胞亚群的单克隆扩增，这是恶性肿瘤的标志。在B细胞淋巴瘤的情况下，单克隆群体的检测基于发现携带一种轻链和重链以及/或一种特定B淋巴细胞分化抗原的细胞类型的单克隆扩增。T细胞恶性肿瘤的诊断更难确立，T细胞淋巴瘤的诊断基于异常表型的发现。因此，流式细胞术与组织形态学检查相结合是一种有用的技术，可用于更精确地诊断淋巴瘤和制定治疗方案。
