受精后海胆卵中果糖1,6 -二磷酸酶活性的变化。

Change in the fructose 1,6-bisphosphatase activity in sea urchin eggs following fertilization.

作者信息

Fujimoto N, Yasumasu I

出版信息

J Biochem. 1979 Sep;86(3):719-24. doi: 10.1093/oxfordjournals.jbchem.a132576.

DOI:10.1093/oxfordjournals.jbchem.a132576

Abstract

The activity of fructose 1,6-bisphosphatase [EC 3.1.3.11] in sea urchin eggs decreased following fertilization. During the first 30 min after fertilization, the activity was considerably lower than that in unfertilized eggs, but by 30 min the activity was similar to that in unfertilized eggs. The enzyme activity in fertilized eggs, estimated in the presence of EGTA, was similar to that in unfertilized eggs. The activity in unfertilized eggs was reduced by Ca2+ at concentrations between 1 X 10(-5) M and 5 X 10(-3) M. Immediately after fertilization, the enzyme was insensitive to concentrations of Ca2+ lower than 2 X 10(-4) M, but the Ca2+ sensitivity of the enzyme recovered 30 min after fertilization. In the presence of Ca2+ at concentrations higher than 2 X 10(-4) M, the enzyme activity in unfertilized eggs was similar to that in fertilized eggs. Mg2+ restored the Ca2+-induced inhibition of fructose 1,6-bisphosphatase. 3-Phosphoglycerate and citrate hardly affected the enzyme activity, and AMP at concentrations above 10 mM inhibited it.

摘要

受精后海胆卵中果糖1,6 - 二磷酸酶[EC 3.1.3.11]的活性降低。在受精后的最初30分钟内，该活性显著低于未受精卵中的活性，但到30分钟时，其活性与未受精卵中的相似。在EGTA存在的情况下测定的受精卵中的酶活性与未受精卵中的相似。未受精卵中的活性在1×10⁻⁵M至5×10⁻³M的Ca²⁺浓度下会降低。受精后立即，该酶对低于2×10⁻⁴M的Ca²⁺浓度不敏感，但受精30分钟后该酶的Ca²⁺敏感性恢复。在高于2×10⁻⁴M的Ca²⁺浓度存在时，未受精卵中的酶活性与受精卵中的相似。Mg²⁺可恢复Ca²⁺诱导的对果糖1,6 - 二磷酸酶的抑制作用。3 - 磷酸甘油酸和柠檬酸盐几乎不影响该酶的活性，而浓度高于10 mM的AMP会抑制它。