海胆卵中3',5'-环磷酸腺苷浓度的周期性变化。
The periodic change in adenosine 3',5'-monophosphate concentration in sea-urchin eggs.
作者信息
Ishida K, Yasumasu I
出版信息
Biochim Biophys Acta. 1982 Jun 8;720(3):266-73. doi: 10.1016/0167-4889(82)90050-7.
The level of adenosine 3',5'-monophosphate (cyclic AMP) in the eggs of the sea urchin, Anthocidaris crassispina, was found to change periodically after fertilization. The minimum and maximum levels of cyclic AMP were 1.0 X 10(-7)M and 1.5 X 10(-6)M, respectively. The activity of adenylate cyclase in a 105 000 X g precipitate reached a plateau at 20 min after fertilization and stayed constant for at least 2 h. It was also found that 1.0 mM CaCl2 increased the activity of adenylate cyclase in the same precipitate from unfertilized eggs. In contrast, phosphodiesterase activity changed periodically and correlated with cyclic AMP levels in the eggs. Up to a concentration of 1.5 X 10(-6)M cyclic AMP, phosphodiesterase activity was low, but it became activated when the level of cyclic AMP rose beyond this level. These results indicate that the change in the intracellular level of cyclic AMP is regulated mainly by the change in phosphodiesterase activity.
研究发现，厚刺海胆（Anthocidaris crassispina）卵中的3',5'-环磷酸腺苷（环磷酸腺苷）水平在受精后会周期性变化。环磷酸腺苷的最低和最高水平分别为1.0×10⁻⁷M和1.5×10⁻⁶M。在105000×g沉淀物中的腺苷酸环化酶活性在受精后20分钟达到平台期，并至少保持2小时恒定。还发现1.0 mM氯化钙可增加未受精卵相同沉淀物中腺苷酸环化酶的活性。相反，磷酸二酯酶活性周期性变化，并与卵中的环磷酸腺苷水平相关。在环磷酸腺苷浓度高达1.5×10⁻⁶M时，磷酸二酯酶活性较低，但当环磷酸腺苷水平超过此水平时，它会被激活。这些结果表明，细胞内环磷酸腺苷水平的变化主要由磷酸二酯酶活性的变化调节。
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