Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, H4P 2R2, Montreal, PQ, Canada.
J Biomol NMR. 1996 Sep;8(2):213-8. doi: 10.1007/BF00211167.
Gradient-enhanced versions of the homonuclear TOCSY experiment are described, with solvent suppression and sensitivity superior to that of a conventional TOCSY experiment. The pulse sequences are constructed by appending a WATERGATE module to a z-filtered TOCSY experiment. Pulsed-field gradients and appropriately phased selective rf pulses are used to maintain precise control of the water magnetization vector. Problems associated with radiation damping and spin-locking of the water magnetization are thus alleviated. The water magnetization is returned to equilibrium prior to each acquisition, which improves water suppression and minimizes signal losses due to saturation transfer.
梯度增强同核 TOCSY 实验的描述，具有溶剂抑制和灵敏度优于传统 TOCSY 实验。脉冲序列通过在 z 过滤的 TOCSY 实验后添加 WATERGATE 模块来构建。使用脉冲场梯度和适当相移的选择性射频脉冲来保持对水磁化矢量的精确控制。因此，减轻了与水磁化辐射阻尼和自旋锁定相关的问题。在每次采集之前，将水磁化恢复到平衡状态，从而提高了水抑制效果，并最大程度地减少了由于饱和转移而导致的信号损失。
