Teixeira F M, Castro L L, Ferreira R T, Pires P A, Vanderlinde F A, Medeiros M A
Departamento de Ciências Fisiológicas, Universidade Federal Rural do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
Braz J Med Biol Res. 2012 Dec;45(12):1209-14. doi: 10.1590/s0100-879x2012007500133. Epub 2012 Aug 23.
The objective of the present study was to compare the effect of electroacupuncture (EA) and carprofen (CP) on postoperative incisional pain using the plantar incision (PI) model in rats. A 1-cm longitudinal incision was made through skin, fascia and muscles of a hind paw of male Wistar rats and the development of mechanical and thermal hypersensitivity was determined over 4 days using the von Frey and Hargreaves methods, respectively. Based on the experimental treatments received on the third postoperative day, the animals were divided into the following groups: PI+CP (CP, 2 mg/kg, po); PI+EAST36 (100-Hz EA applied bilaterally at the Zusanli point (ST36)); PI+EANP (EA applied to a non-acupoint region); PI+IMMO (immobilization only); PI (vehicle). In the von Frey test, the PI+EAST36 group had higher withdrawal force thresholds in response to mechanical stimuli than the PI, PI+IMMO and PI+EANP groups at several times studied. Furthermore, the PI+EAST36 group showed paw withdrawal thresholds in response to mechanical stimuli that were similar to those of the PI+CP group. In the Hargreaves test, all groups had latencies higher than those observed with PI. The PI+EAST36 group was similar to the PI+IMMO, PI+EANP and PI+CP groups. We conclude that 100-Hz EA at the ST36 point, but not at non-acupoints, can reduce mechanical nociception in the rat model of incisional pain, and its effectiveness is comparable to that of carprofen.
本研究的目的是使用大鼠足底切口（PI）模型比较电针（EA）和卡洛芬（CP）对术后切口疼痛的影响。在雄性Wistar大鼠的后爪通过皮肤、筋膜和肌肉做一个1厘米的纵向切口，分别使用von Frey和哈格里夫斯方法在4天内测定机械性和热超敏反应的发展情况。根据术后第三天接受的实验处理，将动物分为以下几组：PI+CP组（CP，2mg/kg，口服）；PI+EAST36组（双侧足三里穴（ST36）施加100Hz电针）；PI+EANP组（电针施加于非穴位区域）；PI+IMMO组（仅固定）；PI组（赋形剂）。在von Frey试验中，在几个研究时间点，PI+EAST36组对机械刺激的撤针力阈值高于PI组、PI+IMMO组和PI+EANP组。此外，PI+EAST36组对机械刺激的爪撤针阈值与PI+CP组相似。在哈格里夫斯试验中，所有组的潜伏期均高于PI组。PI+EAST36组与PI+IMMO组、PI+EANP组和PI+CP组相似。我们得出结论，在ST36穴位而非非穴位施加100Hz电针可减轻大鼠切口疼痛模型中的机械性伤害感受，其效果与卡洛芬相当。
