Department of Comparative Medicine, Stanford University, Stanford, California;, Email:
Department of Mathematics, Naresuan University, Phitsanulok, Thailand.
J Am Assoc Lab Anim Sci. 2020 Mar 1;59(2):163-169. doi: 10.30802/AALAS-JAALAS-19-000093. Epub 2020 Feb 19.
Providing postoperative analgesia to rats by oral administration, compared with injections, reduces stress from frequent handling and is technically easier for investigators. The purpose of this study was to investigate whether bacon-flavored tablets containing gabapentin, carprofen or a combination of both drugs effectively attenuates postoperative mechanical and thermal hypersensitivity in a rat model of incisional pain. Forty-eight Sprague-Dawley rats were randomly assigned to 1 of 5 treatment groups: placebo tablet; a single, subcutaneous injection of buprenorphine sustained release at 1.2 mg/kg; gabapentin 90 mg/tablet; carprofen 5 mg/tablet; gabapentin 90 mg and carprofen 5 mg/tablet (gabapentin/carprofen). Tablets were given to rats on days -3, -2, -1, 0 (surgery), 1, and 2. Rats were anesthetized using isoflurane. A 1 cm skin incision was made aseptically on the plantar surface of the left hindpaw and closed by using suture. Mechanical (von Frey monofilament) and thermal (Hargreaves method) hypersensitivity were tested daily, and analyzed on days -1, 1, 2, and 3. The amount of tablet consumed was recorded daily; postoperatively rats consumed 101 to 133 mg/kg of gabapentin, 5.5 to 5.8 mg/kg of carprofen, and 86-137/1.9-3 mg/kg of gabapentin/carprofen, respectively. Both the gabapentin and carprofen groups displayed attenuated mechanical hypersensitivity on all 3 postsurgical days and decreased thermal hypersensitivity on Day 3. The gabapentin/ carprofen group showed attenuated mechanical hypersensitivity on Day 2 and 3, but no significant reduction of thermal hypersensitivity. These data suggest that both gabapentin and carprofen, given orally by flavored tablet, effectively attenuate postoperative mechanical hypersensitivity for 3 d after surgery in a rat model of incisional pain.
与注射相比，通过口服给予大鼠术后镇痛可以减少频繁处理带来的应激，并且对研究人员来说在技术上更为简单。本研究旨在探讨含有加巴喷丁、卡洛芬或两者联合的培根味片剂是否能有效减轻切口痛大鼠模型术后机械和热痛觉过敏。48 只 Sprague-Dawley 大鼠随机分为 5 个治疗组之一：安慰剂片剂；单次皮下注射 1.2 mg/kg 的丁丙诺啡缓释剂；加巴喷丁 90 mg/片；卡洛芬 5 mg/片；加巴喷丁 90 mg 和卡洛芬 5 mg/片（加巴喷丁/卡洛芬）。片剂在术前 3 天（-3 天）、2 天（-2 天）、1 天（-1 天）、0 天（手术当天）、1 天和 2 天给予大鼠。大鼠用异氟烷麻醉。在左后足的足底表面无菌地做 1 厘米的皮肤切口，并用缝线闭合。每天测试机械（冯弗雷单丝）和热（Hargreaves 法）敏感性，并在术前 1 天、1 天、2 天和 3 天进行分析。每天记录片剂的消耗量；术后大鼠分别消耗 101-133mg/kg 加巴喷丁、5.5-5.8mg/kg 卡洛芬和 86-137/1.9-3mg/kg 加巴喷丁/卡洛芬。加巴喷丁组和卡洛芬组在术后 3 天都显示机械敏感性降低，第 3 天热敏感性降低。加巴喷丁/卡洛芬组在第 2 天和第 3 天显示机械敏感性降低，但热敏感性无明显降低。这些数据表明，在切口痛大鼠模型中，口服调味片剂给予加巴喷丁和卡洛芬均可有效减轻术后 3 天的机械性痛觉过敏。
