Department of Surgery, Far Eastern Memorial Hospital, Taipei, Taiwan.
Ann Surg Oncol. 2013 Feb;20(2):464-73. doi: 10.1245/s10434-012-2593-y. Epub 2012 Aug 22.
Hepatocellular carcinoma (HCC) has a high recurrence rate after resection. Abnormal activation of the sonic hedgehog (SHH) signaling pathway contributing to the carcinogenesis of some organs had been reported. We hypothesize that activation of SHH pathway contributes to the recurrence of HCC after surgical resection.
In a prospective study, from January 2006 to June 2010, a total of 50 consecutive patients with HCC after curative resection were enrolled. The ratio of the expression of messenger RNA (mRNA) of SHH, patched homolog-1 (PTCH-1) and glioma-associated oncogene-1 (GLI-1) between HCC tissues and the paired noncancerous liver tissues were measured. Both the clinicopathologic characteristics and these ratios were compared between those with recurrence and those without recurrence. They were also compared between those with and without survival.
There was a statistically significant correlation among the ratios of PTCH-1 mRNA and serum AFP level (p = 0.045), tumor size (p = 0.001), vascular permeation (p = 0.043) and tumor, node, metastasis staging system (TNM) stage (p = 0.003). A borderline significant correlation was found among ratios of GLI-1 mRNA and tumor size (p = 0.062) and TNM stage (p = 0.051). There was no such significant correlation between SHH mRNA and any parameter. Both the ratios of PTCH-1 mRNA and GLI-1 mRNA significantly adversely affected recurrence (p = 0.003 and 0.005, respectively) and survival (p < 0.001 and <0.001, respectively).
Expression of PTCH-1 mRNA and GLI-1 mRNA in HCC tissues is a potential biomarker to predict postresection disease recurrence.
肝细胞癌（HCC）在切除后复发率很高。据报道，声波刺猬（SHH）信号通路的异常激活有助于一些器官的癌变。我们假设 SHH 通路的激活有助于手术后 HCC 的复发。
在一项前瞻性研究中，2006 年 1 月至 2010 年 6 月，共纳入 50 例根治性切除后 HCC 患者。测量 HCC 组织与配对非癌肝组织中 SHH、 patched homolog-1（PTCH-1）和 glioma-associated oncogene-1（GLI-1）信使 RNA（mRNA）的表达比例。比较有复发和无复发患者的临床病理特征和这些比例。还比较了有和无生存患者之间的这些比例。
PTCH-1mRNA 与血清 AFP 水平（p = 0.045）、肿瘤大小（p = 0.001）、血管渗透（p = 0.043）和肿瘤、淋巴结、转移分期系统（TNM）分期（p = 0.003）之间存在统计学显著相关性。GLI-1mRNA 与肿瘤大小（p = 0.062）和 TNM 分期（p = 0.051）之间存在边缘显著相关性。SHHmRNA 与任何参数之间均无显著相关性。PTCH-1mRNA 和 GLI-1mRNA 的比值均显著影响复发（p = 0.003 和 0.005）和生存（p < 0.001 和 <0.001）。
HCC 组织中 PTCH-1mRNA 和 GLI-1mRNA 的表达是预测术后疾病复发的潜在生物标志物。
