Joukar Siyavash, Sheibani Mohammad, Joukar Farzin
Neuroscience Research Center, Physiology Research Center and Department of Physiology and Pharmacology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Croat Med J. 2012 Aug;53(4):343-9. doi: 10.3325/cmj.2012.53.343.
To investigate whether administration of nifedipine has considerable therapeutic effect in morphine-dependent rats.
Sixty animals were randomized into control, morphine, morphine plus nifedipine, and morphine plus dimethyl sulfoxide (DMSO, as nifedipine soluble) groups. Each group consisted of two subgroups, with and without heart injury. The groups were treated with incremental doses of morphine or morphine plus nifedipine daily for 7 days. Myocardial injury was induced by isoproterenol (50 mg/kg i.p.) on the day 7. On the day 8, the heart rate (HR), blood pressure (BP), rate-pressure product (RPP), and the plasma level of cardiac troponin I were measured and the hearts were histopathologically examined.
In morphine-dependent rats, nifedipine administration was associated with a significantly higher decrease in the plasma level of cardiac troponin I than the administration of morphine alone. This finding was also significant in dependent animals that received only DMSO. HR, BP, RPP, and histopathological indices did not show significant changes in the presence of nifedipine.
Administration of nifedipine failed to show a significant therapeutic effect in morphine-dependent rats, especially in the group with myocardial injury.
研究硝苯地平对吗啡依赖大鼠是否具有显著治疗作用。
将60只动物随机分为对照组、吗啡组、吗啡加硝苯地平组和吗啡加二甲基亚砜（DMSO，作为硝苯地平溶剂）组。每组又分为有心脏损伤和无心脏损伤两个亚组。各组每日递增剂量给予吗啡或吗啡加硝苯地平，持续7天。在第7天，通过腹腔注射异丙肾上腺素（50mg/kg）诱导心肌损伤。在第8天，测量心率（HR）、血压（BP）、速率压力乘积（RPP）以及心肌肌钙蛋白I的血浆水平，并对心脏进行组织病理学检查。
在吗啡依赖大鼠中，与单独给予吗啡相比，给予硝苯地平后心肌肌钙蛋白I的血浆水平显著降低。这一发现在仅接受DMSO的依赖动物中也很显著。在有硝苯地平的情况下，HR、BP、RPP和组织病理学指标未显示出显著变化。
硝苯地平给药对吗啡依赖大鼠未显示出显著治疗作用，尤其是在有心肌损伤的组中。
