香蜂草预处理对心脏抗心肌损伤能力的影响。
The effects of Melissa officinalis (lemon balm) pretreatment on the resistance of the heart to myocardial injury.
作者信息
Joukar Siyavash, Asadipour Haleh, Sheibani Mohammad, Najafipour Hamid, Dabiri Shahriar
机构信息
a Physiology Research Center , Institute of Neuropharmacology, Kerman University of Medical Sciences , Kerman , Iran .
b Department of Physiology and Pharmacology , School of Medicine, Kerman University of Medical Sciences , Kerman , Iran .
出版信息
Pharm Biol. 2016;54(6):1005-13. doi: 10.3109/13880209.2015.1091845. Epub 2015 Oct 12.
CONTEXT
The antihyperlipidemic, antiarrhythmic, neuroprotective and hepatoprotective effects of Melissa officinalis L. (Lamiaceae) have been reported. However, no study has examined its effects on the resistance of the heart to stressful conditions.
OBJECTIVE
The objective of this study is to evaluate the effects of aqueous extract of M. officinalis aerial parts on Wistar rat heart with/without cardiac injury.
MATERIALS AND METHODS
Animals were grouped as control, isoproterenol (ISO), M. officinalis without (M50, M100, and M200) and with isoproterenol (M50 + ISO, M100 + ISO, and M200 + ISO). The aqueous extract of M. officinalis was orally administered at dosages of 50, 100, and 200 mg/kg/d, respectively, for 7 consecutive days. On the 6th and 7th day, ISO, M50 + ISO, M100 + ISO, and M200 + ISO groups received 85 mg/kg of isoproterenol for myocardial injury induction. On day 8, hemodynamic parameters were recorded and samplings were done.
RESULTS
The extract (50, 100, and 200 mg/kg) significantly reduced the heart rate (264 ± 5, 259 ± 5 and 281 ± 3 versus 377 ± 13 in control group, p < 0.01). Blood pressure was significantly decreased in M50 + ISO (75 ± 5) versus M50 (110 ± 6) and M100 + ISO (72 ± 6) versus M100 (105 ± 5 mmHg, p < 0.01). The malondialdehyde levels of the injured hearts were lower in M50 + ISO and M100 + ISO groups than in the ISO group (p < 0.05). Serum cardiac troponin I was higher in the M200 + ISO group (5.1 ± 1.7) than in the ISO group (2.7 ± 0.7 ng/ml, p < 0.05).
CONCLUSION
The lower dose of extract, by improving the balance of the redox system and by reducing the heart rate, may increase the heart resistance to injury. However, the higher doses of extract may intensify the injury of ischemic heart.
背景
已报道过蜜蜂花（唇形科）具有抗高血脂、抗心律失常、神经保护和肝脏保护作用。然而，尚无研究考察其对心脏抵抗应激条件能力的影响。
目的
本研究旨在评估蜜蜂花地上部分水提取物对有/无心脏损伤的Wistar大鼠心脏的影响。
材料与方法
将动物分为对照组、异丙肾上腺素组（ISO）、未用异丙肾上腺素的蜜蜂花组（M50、M100和M200）以及用了异丙肾上腺素的蜜蜂花组（M50 + ISO、M100 + ISO和M200 + ISO）。分别以50、100和200mg/kg/d的剂量口服给予蜜蜂花水提取物，连续7天。在第6天和第7天，ISO组、M50 + ISO组、M100 + ISO组和M200 + ISO组给予85mg/kg异丙肾上腺素以诱导心肌损伤。在第8天，记录血流动力学参数并进行采样。
结果
提取物（50、100和200mg/kg）显著降低心率（分别为264±5、259±5和281±3，对照组为377±13，p<0.01）。M50 + ISO组的血压（75±5）显著低于M50组（110±6），M100 + ISO组的血压（72±6）显著低于M100组（105±5mmHg，p<0.01）。M50 + ISO组和M100 + ISO组损伤心脏的丙二醛水平低于ISO组（p<0.05）。M200 + ISO组的血清心肌肌钙蛋白I（5.1±1.7）高于ISO组（2.7±0.7ng/ml，p<0.05）。
结论
较低剂量的提取物通过改善氧化还原系统平衡和降低心率，可能增加心脏对损伤的抵抗力。然而，较高剂量的提取物可能会加重缺血性心脏的损伤。
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