KU Center for Food Safety, College of Veterinary Medicine, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea.
Poult Sci. 2012 Sep;91(9):2382-5. doi: 10.3382/ps.2011-01980.
In this study, we compared the effectiveness of 2 types of Bolton broths and 3 selective media for isolating Campylobacter spp. from naturally contaminated whole-chicken carcass-rinse samples. One hundred chickens were rinsed with buffered peptone water, and the rinses were added to 2× Bolton broth (with or without blood supplementation). The samples were incubated and then streaked onto Preston agar, modified cefoperazone charcoal deoxycholate agar (mCCDA), and Campy-Cefex agar, which was followed by incubation under microaerobic conditions. No statistical differences were observed (P > 0.05) in isolation rate and selectivity between the 2 types of Bolton broths. Among the 3 selective agars, Preston agar yielded a significantly (P < 0.05) better isolation rate and selectivity. The Campy-Cefex agar, which is recommended by many food authorities for its high quantitative detection ability, showed extensive contamination with competing microorganisms and exhibited the lowest isolation rate and selectivity.
在这项研究中，我们比较了两种 Bolton 肉汤和三种选择性培养基从自然污染的整鸡屠体冲洗样品中分离弯曲菌属的效果。将 100 只鸡用缓冲蛋白胨水冲洗，然后将冲洗液加入 2×Bolton 肉汤（有或没有血液补充）中。将样品孵育，然后划线接种到 Preston 琼脂、改良头孢哌酮活性炭脱胆酸盐琼脂（mCCDA）和 Campy-Cefex 琼脂上，然后在微需氧条件下孵育。两种 Bolton 肉汤的分离率和选择性之间没有统计学差异（P > 0.05）。在三种选择性琼脂中，Preston 琼脂的分离率和选择性显著更好（P < 0.05）。许多食品当局推荐的 Campy-Cefex 琼脂因其具有较高的定量检测能力而受到广泛关注，但它也受到了大量竞争微生物的污染，表现出最低的分离率和选择性。
