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野生型和缺乏苯甲酰胺的拟南芥雄蕊中的多胺稳态。

Polyamine Homeostasis in Wild Type and Phenolamide Deficient Arabidopsis thaliana Stamens.

机构信息

Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry Halle (Saale), Germany.

出版信息

Front Plant Sci. 2012 Aug 17;3:180. doi: 10.3389/fpls.2012.00180. eCollection 2012.

DOI:10.3389/fpls.2012.00180
PMID:22912643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3421149/
Abstract

Polyamines (PAs) like putrescine, spermidine, and spermine are ubiquitous polycationic molecules that occur in all living cells and have a role in a wide variety of biological processes. High amounts of spermidine conjugated to hydroxycinnamic acids are detected in the tryphine of Arabidopsis thaliana pollen grains. Tapetum localized spermidine hydroxycinnamic acid transferase (SHT) is essential for the biosynthesis of these anther specific tris-conjugated spermidine derivatives. Sht knockout lines show a strong reduction of hydroxycinnamic acid amides (HCAAs). The effect of HCAA-deficient anthers on the level of free PAs was measured by a new sensitive and reproducible method using 9-fluorenylmethyl chloroformate (FMOC) and fluorescence detection by HPLC. PA concentrations can be accurately determined even when very limited amounts of plant material, as in the case of A. thaliana stamens, are available. Analysis of free PAs in wild type stamens compared to sht deficient mutants and transcript levels of key PA biosynthetic genes revealed a highly controlled regulation of PA homeostasis in A. thaliana anthers.

摘要

多胺（PAs）如腐胺、亚精胺和精胺是普遍存在的多阳离子分子，存在于所有活细胞中，在各种生物过程中发挥作用。在拟南芥花粉粒的 tryphine 中检测到大量与羟基肉桂酸结合的亚精胺。位于绒毡层的亚精胺羟基肉桂酸转移酶（SHT）是这些花药特异性三共轭亚精胺衍生物生物合成所必需的。Sht 敲除系显示出羟基肉桂酰胺（HCAA）的强烈减少。通过使用 9-芴甲基氯甲酸酯（FMOC）和 HPLC 荧光检测的新的灵敏且可重复的方法测量缺乏 HCAA 的花药对游离 PAs 水平的影响。即使使用非常有限量的植物材料（如拟南芥雄蕊），也可以准确确定 PA 浓度。与 sht 缺陷突变体相比，野生型雄蕊中游离 PAs 的分析以及关键 PA 生物合成基因的转录水平显示出拟南芥花药中 PA 动态平衡的高度控制调节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/48ca98b46bd9/fpls-03-00180-a001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/274c24c35bbb/fpls-03-00180-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/2cbd15249cd9/fpls-03-00180-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/322ea1b3a12d/fpls-03-00180-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/feea1aff17d9/fpls-03-00180-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/45a177e609c5/fpls-03-00180-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/58d2c3999d5a/fpls-03-00180-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/ca2fafeb2694/fpls-03-00180-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/a1a1a5396c33/fpls-03-00180-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/48ca98b46bd9/fpls-03-00180-a001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/274c24c35bbb/fpls-03-00180-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/2cbd15249cd9/fpls-03-00180-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/322ea1b3a12d/fpls-03-00180-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/feea1aff17d9/fpls-03-00180-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/45a177e609c5/fpls-03-00180-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/58d2c3999d5a/fpls-03-00180-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/ca2fafeb2694/fpls-03-00180-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/a1a1a5396c33/fpls-03-00180-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3c4/3421149/48ca98b46bd9/fpls-03-00180-a001.jpg

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