National Research Council Canada, Winnipeg, MB, Canada.
Can J Physiol Pharmacol. 2012 Sep;90(9):1295-301. doi: 10.1139/y2012-094. Epub 2012 Aug 22.
Adipose tissue stromal fraction (ASF) contains multipotent cells capable of differentiation towards several lineages and may be used for the treatment of various degenerative diseases. However, the multipotent cells within ASF have not been fully characterized. In this study we have attempted to characterize stem cells in the ASF obtained through serial dilution. Five single-cell clones were studied. It was found that the single-cell clones exhibited slight but significant differences in proliferative capacity and differentiation potential. We conclude that ASF houses different subtypes of stem cells.
脂肪组织基质部分（ASF）含有多能细胞，能够向多个谱系分化，可用于治疗各种退行性疾病。然而，ASF 中的多能细胞尚未得到充分的表征。在本研究中，我们试图通过连续稀释来对 ASF 中的干细胞进行特征分析。研究了 5 个单细胞克隆。结果发现，单细胞克隆在增殖能力和分化潜能方面表现出微小但显著的差异。我们得出结论，ASF 中存在不同亚型的干细胞。
