Oláh Z, Veselovský J
Klinika Oftalmológie LF UK v Bratislave.
Cesk Slov Oftalmol. 2012 May;68(2):77-81.
In this work the effect by interaction in vitro free amino acid L-arginine.HCl and selected antiglaucomatic drugs prepared mixtures on the physiological IOP values in rabbits is compare.
The experimental works were performed on 5 female rabbits of the New Zealand White species. After instillation 2 drops of in vitro prepared 10% solution of the amino acid L-arginine.HCl with antiglacoma drugs (0.5% Timoptol, 0.005% Xalatan or 2% Trusopt) alone or in double combination mixtures at 8:00 am. into the left conjunctival sac the IOP was measured before and in 15th, 30th, 60th, 120th, 180th, 240th min. and 24 hours after instillation. The right eye was used as control.
The amino acid 10% L-arginine.HCI solution separately decrease the physiologic IOP in rabbit's in confrontation with control eyes -2.9 torr. After antiglaucomatics separetly the decrease of the IOP were constated -0.69 torr by 0.5% Timoptol, -2.1 torr by 0.005% Xalatan, resp. -2.45 torr by 2% Trusopt. 10% L-arginine.HCl with combination of mono antiglaucomatics mixture decrease the physiologic IOP by 0.5% Timoptol -3.32 torr, by 2% Trusopt -4.45 torr, by 0.005% Xalatan only 2.91 torr. Application of 10% L-arginine-HCl in double combination of antiglaumatics decrease the physiologic IOP by 0.5% Timoptol & 0.005% Xalatan -2.96 torr, by 2% Trusopt & 0.005% Xalatan -4.32 torr and COSOPT (fixed combination of 0.5% Timolol maleat & 2% Dorsolamide.HCl) only -1.8 torr.
We assume that the mentioned mixtures were able to decrease the physiologic IOP in rabbits through their better penetration to the receptors of the ciliary body. The mixture of the L-arginine.HCI with antiglaucomaticum Timoptol or Trusopt, resp. double combination of Timoptol & Xalatan or Trusopt & Xalatan was twice as effective as any of the mentioned antiglaucamatic drugs used separately. We conclude that the increased effectivity of the mixtures compared with the separate components is related to the instillation of the by now ready "bioantiglaucomatic".
本研究比较了体外游离氨基酸L-精氨酸盐酸盐与选定的抗青光眼药物制成的混合物对家兔生理眼压值的相互作用效果。
实验选用5只新西兰白兔雌性。上午8点，将2滴体外制备的10%氨基酸L-精氨酸盐酸盐与抗青光眼药物（0.5%噻吗洛尔、0.005%拉坦前列素或2%曲伏前列素）单独或双重组合的混合物滴入左眼结膜囊，在滴入前以及滴入后第15、30、60、120、180、240分钟和24小时测量眼压。右眼作为对照。
与对照眼相比，10%的氨基酸L-精氨酸盐酸盐溶液可使家兔生理眼压降低2.9托。单独使用抗青光眼药物后，眼压降低情况如下：0.5%噻吗洛尔降低0.69托，0.005%拉坦前列素降低2.1托，2%曲伏前列素降低2.45托。10% L-精氨酸盐酸盐与单一抗青光眼药物混合使用时，与0.5%噻吗洛尔混合可使生理眼压降低3.32托，与2%曲伏前列素混合降低4.45托，与0.005%拉坦前列素混合仅降低2.91托。10% L-精氨酸盐酸盐与两种抗青光眼药物双重组合使用时，与0.5%噻吗洛尔和0.005%拉坦前列素混合可使生理眼压降低2.96托，与2%曲伏前列素和0.005%拉坦前列素混合降低4.32托，与可速比（0.5%马来酸噻吗洛尔与2%盐酸多佐胺的固定组合）混合仅降低1.8托。
我们认为上述混合物能够通过更好地渗透到睫状体受体来降低家兔的生理眼压。L-精氨酸盐酸盐与抗青光眼药物噻吗洛尔或曲伏前列素的混合物，以及噻吗洛尔与拉坦前列素或曲伏前列素与拉坦前列素的双重组合，其效果是单独使用上述任何一种抗青光眼药物的两倍。我们得出结论，混合物与单独成分相比效果增强，这与目前已制备好的“生物抗青光眼药物”的滴注有关。
