Oláh Z, Veselovský J, Veselá A
Klinika oftalmológie LF UK, Bratislava, SR.
Cesk Slov Oftalmol. 2007 Jul;63(4):223-9.
To examine the effectivity of the antiglaucomatic Timoptol mixture with amino acid L-taurine.HCl on physiological values of the IOP in rabbits.
The experimental work was performed on 5 female rabbits of the New Zealand White species. Into the left conjunctival sac at 800 was applicated: a) the 10% L-taurine.HCl in 0.5% Timolol; b) the 0.5% Timolol alone; c) the 10% L-taurine.HCl alone. The IOP was measured before and in 5th, 15th, 30th, 60th, 120th, 180th and 240th min. and 24 hours after the instillation. The right eye was used as control.
a) The 10% L-taurine.HCl in 0.5% Timolol showed also significant IOP decrease (without 5th min.) in all measured times with maximum in 180th min. (with value of decrease 6.7 torr) and in 24th hours the decreased showed still significantly lower level - 3.3 torr; b) The antiglaucomatic 0.5% Timolol alone decreased the IOP values compared with the control eye without significance. The IOP values from the 60th min. were nearly linear character (the mean decrease compared with the control eye was only 1 torr); c) The 10% amino acid L-taurine.HCl showed in all measured times only no significant decrease of the physiological IOP.
The results proved that in the mixture of antiglaucomatic Timoptol and amino acid Taurine a new biologically active substance, the bioregulator is created by interaction. This metabolite as "bioantiglaucomatic" showed several times higher effect on the IOP decrease compared with Timolol or Taurine alone. The showed effect on IOP based on interaction of amino acid mixture with antiglaucomatic is specific and efficacy together with duration changed according the type of amino acid.
研究抗青光眼药物噻吗洛尔与氨基酸L - 牛磺酸盐酸盐混合物对兔眼压生理值的有效性。
对5只新西兰白兔雌性进行实验。在8点时，将以下药物滴入左眼结膜囊：a）0.5%噻吗洛尔中加入10% L - 牛磺酸盐酸盐；b）单独使用0.5%噻吗洛尔；c）单独使用10% L - 牛磺酸盐酸盐。在滴入前以及滴入后第5、15、30、60、120、180和240分钟以及24小时测量眼压。右眼作为对照。
a）0.5%噻吗洛尔中加入10% L - 牛磺酸盐酸盐在所有测量时间均显示眼压显著降低（第5分钟除外），在第180分钟时降低幅度最大（降低值为6.7托），在24小时时降低值仍显著较低 - 3.3托；b）单独使用抗青光眼药物0.5%噻吗洛尔与对照眼相比，眼压值降低无显著性差异。从第60分钟起眼压值呈近似线性变化（与对照眼相比平均降低仅1托）；c）10%氨基酸L - 牛磺酸盐酸盐在所有测量时间仅显示生理眼压无显著降低。
结果证明，在抗青光眼药物噻吗洛尔与氨基酸牛磺酸的混合物中，通过相互作用产生了一种新的生物活性物质，即生物调节剂。这种作为“生物抗青光眼药物”的代谢产物对眼压降低的作用比单独使用噻吗洛尔或牛磺酸高出数倍。基于氨基酸混合物与抗青光眼药物相互作用对眼压产生的作用具有特异性，其有效性和持续时间会根据氨基酸类型而改变。
