Dipartimento di Sanità Pubblica e Zootecnia, Facoltà di Medicina Veterinaria, Università degli Studi di Bari, Valenzano, Bari, Italy.
Parasitology. 2013 Jan;140(1):99-108. doi: 10.1017/S0031182012001357. Epub 2012 Aug 23.
Following the recent description of microfilariae of a Cercopithifilaria sp. in a dog from Sicily, Italy, (herein after referred to as Cercopithifilaria sp. I), numerous skin samples were collected from dogs in the Mediterranean region. In addition to Cercopithifilaria sp. I (185·7 ± 7·2 μm long), microfilariae of 2 other species were identified, namely Cercopithifilaria grassii (651·7 ± 23·6 μm long) and a yet undescribed microfilaria, Cercopithifilaria sp. II (264·4 ± 20·2 μm long, with evident lateral alae). The morphological differentiation among the 3 species of dermal microfilariae was confirmed by differences in cytochrome c oxidase subunit 1 and ribosomal 12S sequences examined (mean level of interspecific pairwise distance of 11·4%, and 17·7%, respectively). Phylogenetic analyses were concordant in clustering these with other sequences of Cercopithifilaria spp. to the exclusion of Dirofilaria spp., Onchocerca spp. and Acanthocheilonema spp. Dermal microfilariae collected (n = 132) were morphologically identified as Cercopithifilaria sp. I (n = 108, 81·8%), Cercopithifilaria sp. II (n = 17, 12·9%), whereas only 7 (5·3%) were identified as C. grassii. Mixed infestations were detected in all sites examined. The great diversity of these neglected filarioids in dogs is of biological interest, considering the complex interactions occurring among hosts, ticks and Cercopithifilaria spp. in different environments.
继最近在意大利西西里岛的一只狗中描述了一种塞库皮蒂菲拉利亚（Cercopithifilaria）sp. 的微丝蚴（以下简称 Cercopithifilaria sp. I）后，从地中海地区的犬身上采集了大量皮肤样本。除了 Cercopithifilaria sp. I（长 185.7 ± 7.2 μm）外，还鉴定出了另外 2 种微丝蚴，即 Cercopithifilaria grassii（长 651.7 ± 23.6 μm）和一种尚未描述的微丝蚴 Cercopithifilaria sp. II（长 264.4 ± 20.2 μm，具有明显的侧翅）。通过比较细胞色素 c 氧化酶亚基 1 和核糖体 12S 序列（种间差异平均水平分别为 11.4%和 17.7%）证实了这 3 种皮肤微丝蚴的形态分化。系统发育分析一致地将这些微丝蚴与其他塞库皮蒂菲拉利亚（Cercopithifilaria）种的序列聚类在一起，而将迪罗菲拉利亚（Dirofilaria）种、奥氏属（Onchocerca）种和棘唇属（Acanthocheilonema）种排除在外。收集的皮肤微丝蚴（n = 132）经形态学鉴定为 Cercopithifilaria sp. I（n = 108，81.8%）、Cercopithifilaria sp. II（n = 17，12.9%），而仅有 7 （5.3%）鉴定为 C. grassii。在所有检查的部位都检测到了混合感染。考虑到宿主、蜱和不同环境中的塞库皮蒂菲拉利亚（Cercopithifilaria）种之间发生的复杂相互作用，这些被忽视的线虫在犬中的多样性具有生物学意义。
