通过高效凝胶渗透色谱法对硫酸角质素链分子量进行校准。
Skeletal keratan sulfate chain molecular weight calibration by high-performance gel-permeation chromatography.
作者信息
Dickenson J M, Morris H G, Nieduszynski I A, Huckerby T N
机构信息
Division of Biological Sciences, Lancaster University, Bailrigg, United Kingdom.
出版信息
Anal Biochem. 1990 Nov 1;190(2):271-5. doi: 10.1016/0003-2697(90)90192-c.
A method has been developed for the molecular sizing of skeletal keratan sulfate chains using an HPLC gel-permeation chromatography system. Keratan sulfate chains and keratanase-derived oligosaccharides were prepared from the nucleus pulposus of bovine intervertebral disc (6-year-old animals). A Bio-Gel TSK 30 XL column eluted in 0.2 M NaCl and at 30 degrees C was calibrated with keratan sulfate oligosaccharides of known size as well as 3H-end-labeled keratan sulfate chains to yield the relationship.
已开发出一种使用高效液相色谱凝胶渗透色谱系统对骨骼硫酸角质素链进行分子大小测定的方法。硫酸角质素链和角质酶衍生的寡糖是从牛椎间盘（6岁动物）的髓核中制备的。以已知大小的硫酸角质素寡糖以及3H末端标记的硫酸角质素链对在0.2 M氯化钠中于30℃洗脱的Bio-Gel TSK 30 XL柱进行校准，以得出两者之间的关系。
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