Department of Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin, People's Republic of China.
Inflamm Res. 2013 Jan;62(1):9-15. doi: 10.1007/s00011-012-0545-4. Epub 2012 Aug 23.
Mastitis is defined as inflammation of the mammary gland in domestic dairy animals and humans. Salidroside, a major component isolated from Rhodiola rosea L., has potent anti-inflammatory properties, but whether it can be used in mastitis treatment has not yet been investigated. The aim of this study was to assess the protective effects of salidroside against lipopolysaccharide (LPS)-induced mastitis in mice and the mechanism of action.
We used a mouse mastitis model in which mammary gland inflammation was induced by LPS challenge. Salidroside administered 1 h before LPS infusion significantly attenuated inflammatory cell infiltration, reduced the activity of myeloperoxidase in mammary tissue, and decreased the concentration of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 in a dose-dependent manner. Further studies revealed that salidroside down-regulated phosphorylation of LPS-induced nuclear transcription factor-kappaB (NF-κB) p65 and inhibitor of NF-κB α (IκBα) in the NF-κB signal pathway, and suppressed phosphorylation of p38, extracellular signal-regulated kinase (ERK) and c-jun NH(2)-terminal kinase (JNK) in MAPKs signal pathways.
This study demonstrates that salidroside is an effective suppressor of inflammation and may be a candidate for the prophylaxis of mastitis.
乳腺炎是指家畜和人类乳腺的炎症。红景天苷是从红景天中分离得到的主要成分，具有很强的抗炎作用，但尚未研究其是否可用于乳腺炎的治疗。本研究旨在评估红景天苷对脂多糖（LPS）诱导的乳腺炎小鼠的保护作用及其作用机制。
我们采用 LPS 刺激诱导的小鼠乳腺炎模型。在 LPS 输注前 1 小时给予红景天苷，可显著减轻炎症细胞浸润，降低乳腺组织髓过氧化物酶的活性，并呈剂量依赖性降低肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6 的浓度。进一步的研究表明，红景天苷可下调 LPS 诱导的核转录因子-κB（NF-κB）p65 和 NF-κB 抑制蛋白α（IκBα）在 NF-κB 信号通路中的磷酸化，同时抑制丝裂原活化蛋白激酶（MAPKs）信号通路中 p38、细胞外信号调节激酶（ERK）和 c-jun N 末端激酶（JNK）的磷酸化。
本研究表明，红景天苷是一种有效的炎症抑制剂，可能是预防乳腺炎的候选药物。
